Changes in Serum Thiol-Disulphide Homeostasis in Sheep with Gastrointestinal Nematodes
Elizabeth Moreira dos Santos Schmidt,
Daniele Floriano Fachiolli,
Raphaela Moreira de Oliveira,
Fabiana Alves Almeida,
Cristiano Magalhães Pariz,
Paulo Roberto de Lima Meirelles,
Ciniro Costa,
Asta Tvarijonaviciute,
Ozcan Erel,
Salim Neselioglu,
Jose Joaquin Ceron,
Camila Peres Rubio
Affiliations
Elizabeth Moreira dos Santos Schmidt
Department of Veterinary Clinical Sciences, School of Veterinary Medicine and Animal Science, Campus of Botucatu, São Paulo State University (FMVZ-UNESP), Botucatu 18618-681, Brazil
Daniele Floriano Fachiolli
Department of Animal Nutrition and Breeding, School of Veterinary Medicine and Animal Science, Campus of Botucatu, São Paulo State University (FMVZ-UNESP), Botucatu 18618-681, Brazil
Raphaela Moreira de Oliveira
Department of Veterinary Clinical Sciences, School of Veterinary Medicine and Animal Science, Campus of Botucatu, São Paulo State University (FMVZ-UNESP), Botucatu 18618-681, Brazil
Fabiana Alves Almeida
Department of Parasitology, Biosciences Institute, Campus of Botucatu, São Paulo State University (FMVZ-UNESP), Botucatu 18618-681, Brazil
Cristiano Magalhães Pariz
Department of Animal Nutrition and Breeding, School of Veterinary Medicine and Animal Science, Campus of Botucatu, São Paulo State University (FMVZ-UNESP), Botucatu 18618-681, Brazil
Paulo Roberto de Lima Meirelles
Department of Animal Nutrition and Breeding, School of Veterinary Medicine and Animal Science, Campus of Botucatu, São Paulo State University (FMVZ-UNESP), Botucatu 18618-681, Brazil
Ciniro Costa
Department of Animal Nutrition and Breeding, School of Veterinary Medicine and Animal Science, Campus of Botucatu, São Paulo State University (FMVZ-UNESP), Botucatu 18618-681, Brazil
Asta Tvarijonaviciute
Interdisciplinary Laboratory of Clinical Analysis of the University of Murcia (Interlab-UMU), Regional Campus of International Excellence ‘Campus Mare Nostrum’, University of Murcia, Espinardo, 30100 Murcia, Spain
Ozcan Erel
Department of Biochemistry, Faculty of Medicine, Ankara Yildirim Beyazit University, Ankara City Hospital, Ankara 06800, Turkey
Salim Neselioglu
Department of Biochemistry, Faculty of Medicine, Ankara Yildirim Beyazit University, Ankara City Hospital, Ankara 06800, Turkey
Jose Joaquin Ceron
Interdisciplinary Laboratory of Clinical Analysis of the University of Murcia (Interlab-UMU), Regional Campus of International Excellence ‘Campus Mare Nostrum’, University of Murcia, Espinardo, 30100 Murcia, Spain
Camila Peres Rubio
Department of Veterinary Clinical Sciences, School of Veterinary Medicine and Animal Science, Campus of Botucatu, São Paulo State University (FMVZ-UNESP), Botucatu 18618-681, Brazil
This work aimed to evaluate the thiol-disulphide homeostasis in serum of lambs naturally infected by gastrointestinal nematodes presenting different levels of parasite load indirectly indicated by faecal worm egg counts (EPG). Furthermore, the possible changes in the thiol-disulphide dynamic after different procedures to reduce the parasitic charge, such as the integrated crop-livestock system or anthelmintic treatment, were assessed. The results were compared with a panel of various oxidative stress and inflammatory biomarkers. The lambs were divided into three groups: animals highly infected (EPG higher than 5000) and packed cell volume (PCV) lower than 24% (G1); animals highly infected (EPG higher than 5000) and normal PCV (>24%) (G2); and animals presenting EPG lower than 5000 and normal PCV (>24%) (G3). The highly infected lambs (G1 and G2) showed lower total thiol (TT) and native thiol (SH) (p ≤ 0.01) than those from G3. After treatment, TT and SH increased significantly in all groups (p ≤ 0.01), and the disulphide (SS)/TT and SS/SH ratios decreased significantly (p < 0.01) in G1 and G2. These results show that the thiol-disulphide balance was impaired in lambs infected by gastrointestinal nematodes and that it could be potentially used as a biomarker to monitor this disease.
