Transcriptome analysis for genes involved in fructan biosynthesis in the Jerusalem artichoke (Helianthus tuberosus L.)

Ying Wang; Gui Wang; Zhenjie Zhou; Yuan Zong; Lihui Wang; Shipeng Yang; Yaqi Zhang; Xuemei Sun

doi:10.1080/13102818.2022.2098056

Biotechnology & Biotechnological Equipment (Dec 2022)

Transcriptome analysis for genes involved in fructan biosynthesis in the Jerusalem artichoke (Helianthus tuberosus L.)

Ying Wang,
Gui Wang,
Zhenjie Zhou,
Yuan Zong,
Lihui Wang,
Shipeng Yang,
Yaqi Zhang,
Xuemei Sun

Affiliations

Ying Wang: Qinghai Key Laboratory of Genetics and Physiology of Vegetables, Academy of Agriculture and Forestry Sciences, Qinghai University, Xining, Qinghai, PR China
Gui Wang: Qinghai Key Laboratory of Genetics and Physiology of Vegetables, Academy of Agriculture and Forestry Sciences, Qinghai University, Xining, Qinghai, PR China
Zhenjie Zhou: Qinghai Key Laboratory of Genetics and Physiology of Vegetables, Academy of Agriculture and Forestry Sciences, Qinghai University, Xining, Qinghai, PR China
Yuan Zong: Qinghai Province Key Laboratory of Crop Molecular Breeding, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining, Qinghai, PR China
Lihui Wang: Qinghai Key Laboratory of Genetics and Physiology of Vegetables, Academy of Agriculture and Forestry Sciences, Qinghai University, Xining, Qinghai, PR China
Shipeng Yang: Qinghai Key Laboratory of Genetics and Physiology of Vegetables, Academy of Agriculture and Forestry Sciences, Qinghai University, Xining, Qinghai, PR China
Yaqi Zhang: Qinghai Key Laboratory of Genetics and Physiology of Vegetables, Academy of Agriculture and Forestry Sciences, Qinghai University, Xining, Qinghai, PR China
Xuemei Sun: Qinghai Key Laboratory of Genetics and Physiology of Vegetables, Academy of Agriculture and Forestry Sciences, Qinghai University, Xining, Qinghai, PR China

DOI: https://doi.org/10.1080/13102818.2022.2098056
Journal volume & issue: Vol. 36, no. 1
pp. 472 – 480

Abstract

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AbstractJerusalem artichoke (Helianthus tuberosus L.) is a fructan-accumulating plant and an industrial raw material for fructan production. However, the genetic mechanism of fructan biosynthesis remains unclear. Therefore, this study performed transcriptome analysis to determine the genetic differences between two Jerusalem artichoke genotypes and different fructan contents. Approximately 19.73 Gb of clean data were obtained after filtering, and a total of 164,006 unigenes were annotated based on GO, KOG and KEGG functional classification. Then, seven homologous genes involved in fructan biosynthesis were obtained by homology comparison. CL11458.Contig2 and CL7122.Contig14 were candidate genes for sucrose:sucrose 1-fructosyl-transferase (1-SST) and fructan:fructan 1-fructosyl-transferase (1-FFT), respectively. The genetic relationship between SST and SST from artichoke in the phylogenetic tree are relatively close, and FFT is located in the same branch as FFT from ‘Qingyu No.1’ Jerusalem artichoke and Viguiera. SST and FFT were the main regulatory genes of fructan accumulation in Jerusalem artichoke. Therefore, this study successfully identified major genes involved in fructan biosynthesis. Future studies should aim to prove the role of each candidate gene in fructan biosynthesis in Jerusalem artichoke.

Published in Biotechnology & Biotechnological Equipment

ISSN: 1310-2818 (Print); 1314-3530 (Online)
Publisher: Taylor & Francis Group
Country of publisher: United Kingdom
LCC subjects: Technology: Chemical technology: Biotechnology
Website: https://www.tandfonline.com/journals/tbeq

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