Circular RNA CircPRMT5 Accelerates Proliferation and Invasion of Papillary Thyroid Cancer Through Regulation of miR-30c/E2F3 Axis

Abstract

Cheng Xue,1 Yi Cheng,1 Jinyou Wu,1 Kongliang Ke,2 Chundi Miao,2 Enfu Chen,1 Luqing Zhang2 1Department of Endocrinology, Wenling First People’s Hospital, Wenling 317500, People’s Republic of China; 2Department of Anorectal Surgery, Ningbo Hangzhou Bay Hospital, Ningbo 315000, People’s Republic of ChinaCorrespondence: Luqing ZhangDepartment of Anorectal Surgery, Ningbo Hangzhou Bay Hospital, 1155 Binhai 2nd Road, Hangzhouwan New District, Ningbo 315000, People’s Republic of ChinaEmail [email protected]: The role of circular RNA (circRNA) in papillary thyroid cancer (PTC) is largely unknown. This study aims to determine the function and mechanism of circPRMT5 in the regulation of PTC development.Methods: PTC tissues and cell lines were used to determine circPRMT5 expression via quantitative real-time polymerase chain reaction. Small interfering RNA (siRNA) was utilized to knock down circPRMT5. Proliferation was analyzed through CCK8 and colony formation assays. Transwell assay was performed to determine cell migration and invasion. Luciferase assay and RIP assay were carried out to analyze the interaction between circPRMT5 and miR-30c.Results: CircPRMT5 expression was upregulated in PTC tissues and cell lines. And circPRMT5 level was positively linked with advanced stage and lymph node metastasis. CircPRMT5 knockdown inhibited proliferation, migration and invasion while inducing apoptosis. CircPRMT5 worked as a competing endogenous RNA for miR-30c. By inhibiting miR-30c, circPRMT5 promoted the expression of E2F3.Conclusion: Our findings demonstrate that circPRMT5 acts as an oncogenic circRNA to promote PTC progression via regulating miR-30c/E2F3 axis.Keywords: circRNA, papillary thyroid cancer, circPRMT5, miR-30c, E2F3

Keywords

• circrna
• papillary thyroid cancer
• circprmt5
• mir-30c
• e2f3