Frontiers in Genetics (Jul 2021)

A New Type of Allodiploid Hybrids Derived From Female Megalobrama amblycephala × Male Gobiocypris rarus

  • Qingfeng Liu,
  • Qingfeng Liu,
  • Xuanyi Zhang,
  • Xuanyi Zhang,
  • Junmei Liu,
  • Junmei Liu,
  • Fanglei Liu,
  • Fanglei Liu,
  • Fangming Shi,
  • Qinbo Qin,
  • Qinbo Qin,
  • Min Tao,
  • Min Tao,
  • Chenchen Tang,
  • Chenchen Tang,
  • Shaojun Liu,
  • Shaojun Liu

DOI
https://doi.org/10.3389/fgene.2021.685914
Journal volume & issue
Vol. 12

Abstract

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Distant hybridization can combine whole genomes from parent species and result in changes in the phenotypes and genotypes in hybrids. The characteristics of many hybrid fishes with even number of chromosomes have been reported, but the hybrids with odd number chromosomes are rarely reported. Blunt snout bream (Megalobrama amblycephala, BSB, 2n = 48) and rare gudgeon (Gobiocypris rarus, RG, 2n = 50) belong to two different subfamilies and have quite different biological characteristics. In this study, we obtain the hybrids (BR) derived from the inter-subfamily hybridization of female BSB and male RG. We investigate the fertilization rate, hatching rate, morphological traits, chromosomal numbers, DNA content, growth rates, and 5S rDNA in the BR. The results show that the BR is an allodiploid fish with 49 chromosomes, and all the measurable traits are significantly different (p < 0.05) among BR, BSB, and BR. Interestingly, the upper part of the BR body color is similar to BSB (gray), the lower part of the BR body color is similar to RG (light yellow), and the BR inherits a unique light yellow wide longitudinal band from the RG. Furthermore, the BR has a fast growth rate compared with RG. The 5S rDNA of the BR inherits the specific bands of its parental 5S rDNA respectively and has some mutations, which show obvious recombination, heredity, and variability in BR. This study will be of great significance in fish genetic breeding.

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