Chemical Composition and Anti-Urolithiatic Activity of Extracts from <i>Argania spinosa</i> (L.) Skeels Press-Cake and <i>Acacia senegal</i> (L.) Willd
Fatima Ezzahra El oumari,
Dalila Bousta,
Hamada Imtara,
Anissa Lahrichi,
Radouane Elhabbani,
Ghita El mouhri,
Omkulthom Al kamaly,
Asmaa Saleh,
Mohammad Khalid Parvez,
Andriy Grafov,
Tarik Sqalli Houssaini
Affiliations
Fatima Ezzahra El oumari
Laboratory of Epidemiology and Research in Health Sciences, Faculty of Medicine and Pharmacy, Dental Medicine University of Sidi Mohammed Ben Abdellah, Fez 30070, Morocco
Dalila Bousta
Laboratory of Biotechnology, Environment, Agri-Food, and Health (LBEAS), Faculty of Sciences, University of Sidi Mohamed Ben Abdellah, Fez 30050, Morocco
Hamada Imtara
Faculty of Arts and Sciences, Arab American University Palestine, P.O. Box 240, Jenin 44862, Palestine
Anissa Lahrichi
Laboratory of Biochemistry, Faculty of Medicine and Pharmacy, Dental Medicine Sidi Mohammed Ben Abdellah University, BP 1893, Km 22, Road of Sidi Harazem, Fez 30070, Morocco
Radouane Elhabbani
Laboratory of Epidemiology and Research in Health Sciences, Faculty of Medicine and Pharmacy, Dental Medicine University of Sidi Mohammed Ben Abdellah, Fez 30070, Morocco
Ghita El mouhri
Laboratory of Biochemistry, Faculty of Medicine and Pharmacy, Dental Medicine Sidi Mohammed Ben Abdellah University, BP 1893, Km 22, Road of Sidi Harazem, Fez 30070, Morocco
Omkulthom Al kamaly
Department of Pharmaceutical Sciences, College of Pharmacy, Princess Nourah bint Abdulrahman University, P.O. Box 84428, Riyadh 11671, Saudi Arabia
Asmaa Saleh
Department of Pharmaceutical Sciences, College of Pharmacy, Princess Nourah bint Abdulrahman University, P.O. Box 84428, Riyadh 11671, Saudi Arabia
Mohammad Khalid Parvez
Department of Pharmacognosy, College of Pharmacy King Saud University, P.O. Box 2457,
Riyadh 11451, Saudi Arabia
Andriy Grafov
Department of Chemistry, University of Helsinki, P.O. Box 55, FI-00014 Helsinki, Finland
Tarik Sqalli Houssaini
Laboratory of Epidemiology and Research in Health Sciences, Faculty of Medicine and Pharmacy, Dental Medicine University of Sidi Mohammed Ben Abdellah, Fez 30070, Morocco
Ethnobotanical studies have reported the traditional medicinal uses of Acacia senegal (L.) Willd. and Argania spinosa (L.) Skeels against kidney stone formation and other chronic kidney diseases. The present work is undertaken to study the litholytic activity and the inhibiting activity of calcium oxalate crystallization by bioactive compounds identified in Argania spinosa (L.) Skeels press-cake (residue of Argan oil) and in Acacia senegal (L.) Willd. The litholytic activity was studied in vitro on cystine and uric acid stones using a porous bag and an Erlenmeyer glass. The study of the inhibiting activity of calcium oxalate crystallization, was based on temporal measurements of the optical density, registered at a 620 nm wavelength for 30 min using an ultraviolet–visible spectrophotometer. The silylation method was performed to identify phytochemicals, followed by gas chromatography coupled with mass spectrophotometry (GC/MS) analysis. The results show significant litholytic activity of Argania Spinosa press-cake hydro-ethanolic extract on uric acid and cystine stones, respectively, with dissolution rates (DR) of 86.38% and 60.42% versus 3.23% and 9.48% for the hydro-ethanolic extract of Acacia senegal exudate. Furthermore, the percentages of nucleation inhibition are 83.78% and 43.77% (p ˂ 0.05) for Argania spinosa and Acacia senegal, respectively. The results point to the detection of 17 phytochemicals in Argania spinosa press-cake extract, the majority of which are phenolic acids and have potent anti-urolithiatic action.
