The novel genomic DNA integrity in neutral buffer formalin, Bouin’s fluid, and zinc formalin fixatives: A comparative study

Abstract

DNA extraction was successfully performed on all samples with variable concentrations. Fifteen liver samples were subjected to the extraction technique, with five liver samples utilized from each fixative, this comparison study aimed to assess DNA integrity across different fixatives. DNA was successfully extracted from all samples regardless of their varying quantities. Each sample yielded at least one motif with a length of 100 base pairs. All 15 samples produced 100 bp amplicon for the IRBP gene. All 15 samples generated a 200 bp amplicon from the NBF, ZF, and BF. The success proportion dropped with growing amplicon length, by which 66.6% (10/15) of 300 bp amplicons were successfully produced from NBF and ZF, showing significant differences at P<0.05 than BF, while no other amplicon length obtained 0% for 400, 500, and 750 bp respectively, on the other hand, frozen samples worked as a control were able to produce all amplicon length from 100bp to 750bp. Only samples from NBF and ZF constantly yielded more extensive amplicon ranges than BF. BF was unable to keep intact DNA fragments of more than 200 bp. Our study concluded that different fixatives could produce at least 100 bp and 200 bp. However, ZF and NBF are more dependable than BF in producing 300 bp for downstream analysis for further next-generation techniques. Furthermore, NBF and ZF are supreme in producing better-quality DNA fragments than BF.

Keywords

• dna integrity
• neutral buffer formalin
• zinc formalin
• bouin’s fluid
• amplicon