Genome-Wide Identification and Expression Analysis of the <i>PsTPS</i> Gene Family in <i>Pisum sativum</i>
Hao Yuan,
Baoxia Liu,
Guwen Zhang,
Zhijuan Feng,
Bin Wang,
Yuanpeng Bu,
Yu Xu,
Zhihong Sun,
Na Liu,
Yaming Gong
Affiliations
Hao Yuan
State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-Products, Ministry of Agriculture and Rural Affairs Key Laboratory of Vegetable Legumes Germplasm Enhancement and Molecular Breeding in Southern China, Institute of Vegetables, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China
Baoxia Liu
State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-Products, Ministry of Agriculture and Rural Affairs Key Laboratory of Vegetable Legumes Germplasm Enhancement and Molecular Breeding in Southern China, Institute of Vegetables, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China
Guwen Zhang
State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-Products, Ministry of Agriculture and Rural Affairs Key Laboratory of Vegetable Legumes Germplasm Enhancement and Molecular Breeding in Southern China, Institute of Vegetables, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China
Zhijuan Feng
State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-Products, Ministry of Agriculture and Rural Affairs Key Laboratory of Vegetable Legumes Germplasm Enhancement and Molecular Breeding in Southern China, Institute of Vegetables, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China
Bin Wang
State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-Products, Ministry of Agriculture and Rural Affairs Key Laboratory of Vegetable Legumes Germplasm Enhancement and Molecular Breeding in Southern China, Institute of Vegetables, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China
Yuanpeng Bu
State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-Products, Ministry of Agriculture and Rural Affairs Key Laboratory of Vegetable Legumes Germplasm Enhancement and Molecular Breeding in Southern China, Institute of Vegetables, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China
Yu Xu
State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-Products, Ministry of Agriculture and Rural Affairs Key Laboratory of Vegetable Legumes Germplasm Enhancement and Molecular Breeding in Southern China, Institute of Vegetables, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China
Zhihong Sun
College of Horticulture Science, Zhejiang A&F University, Hangzhou 311300, China
Na Liu
State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-Products, Ministry of Agriculture and Rural Affairs Key Laboratory of Vegetable Legumes Germplasm Enhancement and Molecular Breeding in Southern China, Institute of Vegetables, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China
Yaming Gong
State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-Products, Ministry of Agriculture and Rural Affairs Key Laboratory of Vegetable Legumes Germplasm Enhancement and Molecular Breeding in Southern China, Institute of Vegetables, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China
This study aimed to explore the role of the trehalose-6-phosphate synthase (TPS) gene family in the adaptation of peas to environmental stress. A comprehensive analysis of the PsTPS gene family identified 20 genes with conserved domains and specific chromosomal locations. Phylogenetic analysis delineated evolutionary relationships, while gene structure analysis revealed compositional insights, and motif analysis provided functional insights. Cis-regulatory element identification predicted gene regulation patterns. Tissue-specific and stress-induced expression profiling highlighted eight genes with ubiquitous expression, with PsTPS15 and PsTPS18 displaying elevated expression levels in roots, nodules, and young stems, and PsTPS13 and PsTPS19 expression downregulated in seeds. Transcriptome analysis identified a differential expression of 20 PsTPS genes, highlighting the significance of 14 genes in response to drought and salinity stress. Notably, under drought conditions, the expression of PsTPS4 and PsTPS6 was initially upregulated and then downregulated, whereas that of PsTPS15 and PsTPS19 was downregulated. Salinity stress notably altered the expression of PsTPS4, PsTPS6, and PsTPS19. Taken together, these findings elucidate the regulatory mechanisms of the PsTPS gene family and their potential as genetic targets for enhancing crop stress tolerance.
