Association of colistin residues and manure treatment with the abundance of mcr-1 gene in swine feedlots

Xi Xia; Zheng Wang; Yulin Fu; Xiang-dang Du; Binwen Gao; Yuqing Zhou; Junjia He; Yang Wang; Jianzhong Shen; Haiyang Jiang; Yongning Wu

Environment International (Jun 2019)

Association of colistin residues and manure treatment with the abundance of mcr-1 gene in swine feedlots

Xi Xia,
Zheng Wang,
Yulin Fu,
Xiang-dang Du,
Binwen Gao,
Yuqing Zhou,
Junjia He,
Yang Wang,
Jianzhong Shen,
Haiyang Jiang,
Yongning Wu

Affiliations

Xi Xia: Beijing Advanced Innovation Center for Food Nutrition and Human Health, College of Veterinary Medicine, China Agricultural University, Beijing, China
Zheng Wang: Beijing Advanced Innovation Center for Food Nutrition and Human Health, College of Veterinary Medicine, China Agricultural University, Beijing, China
Yulin Fu: Beijing Advanced Innovation Center for Food Nutrition and Human Health, College of Veterinary Medicine, China Agricultural University, Beijing, China
Xiang-dang Du: College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou, China
Binwen Gao: Institute of Animal Husbandry and Veterinary Science, Henan Academy of Agricultural Sciences, Zhengzhou, China
Yuqing Zhou: Beijing Advanced Innovation Center for Food Nutrition and Human Health, College of Veterinary Medicine, China Agricultural University, Beijing, China
Junjia He: Beijing Advanced Innovation Center for Food Nutrition and Human Health, College of Veterinary Medicine, China Agricultural University, Beijing, China
Yang Wang: Beijing Advanced Innovation Center for Food Nutrition and Human Health, College of Veterinary Medicine, China Agricultural University, Beijing, China
Jianzhong Shen: Beijing Advanced Innovation Center for Food Nutrition and Human Health, College of Veterinary Medicine, China Agricultural University, Beijing, China
Haiyang Jiang: Beijing Advanced Innovation Center for Food Nutrition and Human Health, College of Veterinary Medicine, China Agricultural University, Beijing, China; Correspondence to: H. Jiang, College of Veterinary Medicine, China Agricultural University, 2 Yuanmingyuan West Road, Beijing 100193, China.
Yongning Wu: NHC Key Laboratory of Food Safety Risk Assessment, China National Center for Food Safety Risk Assessment, Beijing, China; Correspondence to: Y. Wu, NHC Key Laboratory of Food Safety Risk Assessment, China National Center for Food Safety Risk Assessment, 7 Panjiayuan Nanli, Beijing 100021, China.

Journal volume & issue: Vol. 127
pp. 361 – 370

Abstract

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Background: The extensive use of colistin in swine production may have contributed to the recent emergence of corresponding mobile resistance gene mcr-1. The use of colistin as a feed additive was banned in China in April 2017. Objectives: To examine the occurrence of colistin and dissemination of mcr-1 in swine feedlots before and after the colistin ban and effects of different manure treatments. Methods: Environmental samples were collected from swine feedlots before (December 2016) and after (December 2017) the colistin ban. Colistin concentrations were determined by ultra-high performance liquid chromatography coupled to tandem mass spectrometry. The prevalence of mcr-1 were determined by quantitative PCR analysis, while bacterial community composition was investigated by 16S rRNA sequencing. Results: In 2016, colistin was detected in feed and fresh manure samples at 67 mg/kg and 17 mg/kg, respectively, but was absent from all samples in 2017. In 2016, the relative abundance of mcr-1 in fresh manure was lower than that in solid samples after natural drying, while a higher relative abundance was detected in fresh manure samples compared with biogas slurry samples. A strong correlation between colistin concentration and relative abundance of mcr-1 was observed in fresh manure. The samples collected in 2017 showed a lower relative abundance of mcr-1 compared with those collected in 2016. Bacterial community analysis showed that the abundance of Enterobacteriaceae, which act as a vehicle and reservoir of mcr-1, increased with natural dying but decreased with anaerobic digestion. Conclusions: The presence of colistin exerts direct selection pressure for the accumulation of mcr-1 in manure, while the ban on colistin likely halted the dissemination of mcr-1 on pig farms. Anaerobic digestion is an effective waste treatment process for removing mcr-1, which might be mainly driven by the shift in bacterial community structure. Keywords: Colistin, mcr-1 gene, Wastewater treat process, Swine feedlot, Bacterial community

Published in Environment International

ISSN: 0160-4120 (Print)
Publisher: Elsevier
Country of publisher: United Kingdom
LCC subjects: Geography. Anthropology. Recreation: Environmental sciences
Website: https://www.journals.elsevier.com/environment-international

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