Marine Cyclotripeptide X-13 Promotes Angiogenesis in Zebrafish and Human Endothelial Cells via PI3K/Akt/eNOS Signaling Pathways

Abstract

Cyclotripeptide X-13 is a core of novel marine compound xyloallenoide A isolated from mangrove fungus <em>Xylaria</em> sp. (no. 2508). We found that X-13 dose-dependently induced angiogenesis in zebrafish embryos and in human endothelial cells, which was accompanied by increased phosphorylation of eNOS and Akt and NO release. Inhibition of PI3K/Akt/eNOS by LY294002 or l-NAME suppressed X-13-induced angiogenesis. The present work demonstrates that X-13 promotes angiogenesis via PI3K/Akt/eNOS pathways.

Keywords

• angiogenesis
• marine cyclotripeptide
• zebrafish
• endothelial nitric oxide synthase
• xyloallenoide A