Green synthesis of AgNPs using plant extract and investigation of its anti-human colorectal cancer application

Abstract

Recently, the plant extracts used to synthesize nanoparticles (NPs) have been considered an excellent alternative to physical and chemical ways. The applications of NPs in the fields of agriculture, industry, and medicine are so many and diverse that they cannot be counted. In recent years, silver nanoparticles (AgNPs) have attracted the consideration of several scientists because of their special characteristics and many applications in various fields, including optoelectronic catalysts, biological markers, and pharmaceutical and medical applications. In the current experiment, the cytotoxic potential of the properties of AgNPs green formulation using green tea on human colorectal cancer cells were determined. The NPs characterization was done by field emission-scanning electron microscopes, Fourier transform infrared spectroscopy, and X-ray diffraction analysis. The average diameter of the particles was about 35 nm. The presence of (111), (200), (220), and (311) peaks at the positions of 38°, 44°, 63°, and 77° indicate the presence of AgNPs, which confirms the correct synthesis of AgNPs. The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay was used to measure anti-colorectal carcinoma (on HCT-8, HT-29, MDST8, HCA-7 Colony 29, HCT 116, and Ramos.2G6.4C10 cells) properties of AgNPs. The findings indicate that in 3 days, the cancer cell survival percentage in various dilations reduced as much as the NPs concentration increased. The best anticancer effect was reported at 1,000 μg/mL dilation. The IC50 was 141, 46, 149, 125, 125, and 44 µg/mL against HCT-8, HT-29, MDST8, HCA-7 Colony 29, HCT 116, and Ramos.2G6.4C10 colorectal cancer cells, respectively. The results indicated that these NPs could inhibit colorectal cancer cells more strongly than normal cells. After doing the clinical trial studies, the recent AgNPs are a suitable option for colorectal cancer treatment.

Keywords

• cancer cell lines
• colorectal cancer
• mtt assay
• silver nanoparticles