A TEMPOL and rapamycin loaded nanofiber-covered stent favors endothelialization and mitigates neointimal hyperplasia and local inflammation

Rui Wang; Jian Lu; Jiasheng Yin; Han Chen; Hongmei Liu; Fei Xu; Tongtong Zang; Rende Xu; Chenguang Li; Yizhe Wu; Qilin Wu; Xiang Fei; Meifang Zhu; Li Shen; Junbo Ge

Bioactive Materials (Jan 2023)

A TEMPOL and rapamycin loaded nanofiber-covered stent favors endothelialization and mitigates neointimal hyperplasia and local inflammation

Rui Wang,
Jian Lu,
Jiasheng Yin,
Han Chen,
Hongmei Liu,
Fei Xu,
Tongtong Zang,
Rende Xu,
Chenguang Li,
Yizhe Wu,
Qilin Wu,
Xiang Fei,
Meifang Zhu,
Li Shen,
Junbo Ge

Affiliations

Rui Wang: Department of Cardiology, Zhongshan Hospital, Fudan University, Shanghai Institute of Cardiovascular Diseases, National Clinical Research Center for Interventional Medicine, Shanghai, China; Research Unit of Cardiovascular Techniques and Devices, Chinese Academy of Medical Sciences, Shanghai, China
Jian Lu: State Key Laboratory for Modification of Chemical Fibers and Polymer Materials, College of Materials Science and Engineering, International Joint Laboratory for Advanced Fiber and Low-dimension Materials, Donghua University, Shanghai, 201620, China
Jiasheng Yin: Department of Cardiology, Zhongshan Hospital, Fudan University, Shanghai Institute of Cardiovascular Diseases, National Clinical Research Center for Interventional Medicine, Shanghai, China; Research Unit of Cardiovascular Techniques and Devices, Chinese Academy of Medical Sciences, Shanghai, China
Han Chen: Department of Cardiology, Zhongshan Hospital, Fudan University, Shanghai Institute of Cardiovascular Diseases, National Clinical Research Center for Interventional Medicine, Shanghai, China; Research Unit of Cardiovascular Techniques and Devices, Chinese Academy of Medical Sciences, Shanghai, China
Hongmei Liu: State Key Laboratory for Modification of Chemical Fibers and Polymer Materials, College of Materials Science and Engineering, International Joint Laboratory for Advanced Fiber and Low-dimension Materials, Donghua University, Shanghai, 201620, China
Fei Xu: Department of Cardiology, Zhongshan Hospital, Fudan University, Shanghai Institute of Cardiovascular Diseases, National Clinical Research Center for Interventional Medicine, Shanghai, China; Research Unit of Cardiovascular Techniques and Devices, Chinese Academy of Medical Sciences, Shanghai, China
Tongtong Zang: Department of Cardiology, Zhongshan Hospital, Fudan University, Shanghai Institute of Cardiovascular Diseases, National Clinical Research Center for Interventional Medicine, Shanghai, China; Research Unit of Cardiovascular Techniques and Devices, Chinese Academy of Medical Sciences, Shanghai, China
Rende Xu: Department of Cardiology, Zhongshan Hospital, Fudan University, Shanghai Institute of Cardiovascular Diseases, National Clinical Research Center for Interventional Medicine, Shanghai, China; Research Unit of Cardiovascular Techniques and Devices, Chinese Academy of Medical Sciences, Shanghai, China; Shanghai Engineering Research Center of Interventional Medicine, Shanghai, China
Chenguang Li: Department of Cardiology, Zhongshan Hospital, Fudan University, Shanghai Institute of Cardiovascular Diseases, National Clinical Research Center for Interventional Medicine, Shanghai, China; Research Unit of Cardiovascular Techniques and Devices, Chinese Academy of Medical Sciences, Shanghai, China; Shanghai Engineering Research Center of Interventional Medicine, Shanghai, China
Yizhe Wu: Department of Cardiology, Zhongshan Hospital, Fudan University, Shanghai Institute of Cardiovascular Diseases, National Clinical Research Center for Interventional Medicine, Shanghai, China; Research Unit of Cardiovascular Techniques and Devices, Chinese Academy of Medical Sciences, Shanghai, China; Shanghai Engineering Research Center of Interventional Medicine, Shanghai, China
Qilin Wu: State Key Laboratory for Modification of Chemical Fibers and Polymer Materials, College of Materials Science and Engineering, International Joint Laboratory for Advanced Fiber and Low-dimension Materials, Donghua University, Shanghai, 201620, China
Xiang Fei: State Key Laboratory for Modification of Chemical Fibers and Polymer Materials, College of Materials Science and Engineering, International Joint Laboratory for Advanced Fiber and Low-dimension Materials, Donghua University, Shanghai, 201620, China; Corresponding author.
Meifang Zhu: State Key Laboratory for Modification of Chemical Fibers and Polymer Materials, College of Materials Science and Engineering, International Joint Laboratory for Advanced Fiber and Low-dimension Materials, Donghua University, Shanghai, 201620, China; Corresponding author.
Li Shen: Department of Cardiology, Zhongshan Hospital, Fudan University, Shanghai Institute of Cardiovascular Diseases, National Clinical Research Center for Interventional Medicine, Shanghai, China; Research Unit of Cardiovascular Techniques and Devices, Chinese Academy of Medical Sciences, Shanghai, China; Shanghai Engineering Research Center of Interventional Medicine, Shanghai, China; Corresponding author. Department of Cardiology, Zhongshan Hospital, Fudan University, Shanghai Institute of Cardiovascular Diseases, National Clinical Research Center for Interventional Medicine, Shanghai, China.
Junbo Ge: Department of Cardiology, Zhongshan Hospital, Fudan University, Shanghai Institute of Cardiovascular Diseases, National Clinical Research Center for Interventional Medicine, Shanghai, China; Research Unit of Cardiovascular Techniques and Devices, Chinese Academy of Medical Sciences, Shanghai, China; Shanghai Engineering Research Center of Interventional Medicine, Shanghai, China; Corresponding author. Department of Cardiology, Zhongshan Hospital, Fudan University, Shanghai Institute of Cardiovascular Diseases, National Clinical Research Center for Interventional Medicine, Shanghai, China.

Journal volume & issue: Vol. 19
pp. 666 – 677

Abstract

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An increased level of reactive oxygen species (ROS) plays a major role in endothelial dysfunction and vascular smooth muscle cell (VSMC) proliferation during in-stent thrombosis and restenosis after coronary artery stenting. Herein, we report an electrospun core-shell nanofiber coloaded with 4-hydroxy-2,2,6,6-tetramethylpiperidine 1-oxyl (TEMPOL) and rapamycin (RAPA) that correspondingly serves as an ROS scavenger and VSMC inhibitor. This system has the potential to improve the biocompatibility of current drug-eluting stent (DES) coatings with the long-term and continuous release of TEMPOL and rapamycin. Moreover, the RAPA/TEMPOL-loaded membrane selectively inhibited the proliferation of VSMCs while sparing endothelial cells (ECs). This membrane demonstrated superior ROS-scavenging, anti-inflammatory and antithrombogenic effects in ECs. In addition, the membrane could maintain the contractile phenotype and mitigate platelet-derived growth factor BB (PDGF-BB)-induced proliferation of VSMCs. In vivo results further revealed that the RAPA/TEMPOL-loaded covered stents promoted rapid restoration of vascular endothelium compared with DES and persistently impeded inflammation and neointimal hyperplasia in porcine models.

Published in Bioactive Materials

ISSN: 2452-199X (Online)
Publisher: KeAi Communications Co., Ltd.
Country of publisher: China
LCC subjects: Technology: Electrical engineering. Electronics. Nuclear engineering: Materials of engineering and construction. Mechanics of materials; Science: Biology (General)
Website: https://www.keaipublishing.com/en/journals/bioactive-materials/

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