Monitoring of aflatoxin M1 in raw cow milk in Croatia during winter 2015
Nina Bilandžić,
Ivana Varenina,
Božica Solomun Kolanović,
Đurđica Božić Luburić,
Miroslav Benić,
Luka Cvetnić,
Sanin Tanković,
Željko Cvetnić
Affiliations
Nina Bilandžić
Department of Veterinary Public Health, Laboratory for Residue Control, Croatian Veterinary Institute, Zagreb, Croatia
Ivana Varenina
Department of Veterinary Public Health, Laboratory for Residue Control, Croatian Veterinary Institute, Zagreb, Croatia
Božica Solomun Kolanović
Department of Veterinary Public Health, Laboratory for Residue Control, Croatian Veterinary Institute, Zagreb, Croatia
Đurđica Božić Luburić
Department of Veterinary Public Health, Laboratory for Residue Control, Croatian Veterinary Institute, Zagreb, Croatia
Miroslav Benić
Laboratory for Mastitis and Raw Milk Quality, Department for Bacteriology and Parasites, Croatian Veterinary Institute, Zagreb, Croatia
Luka Cvetnić
Faculty of Veterinary Medicine, University of Zagreb, Zagreb, Croatia
Sanin Tanković
Veterinary Office of Bosnia and Herzegovina, Sarajevo, Bosnia and Herzegovina
Željko Cvetnić
Laboratory for Bacterial Zoonoses and Molecular Diagnostics of Bacterial Diseases, Department for Bacteriology and Parasites, Croatian Veterinary Institute, Zagreb, Croatia
A total of 548 raw milk samples were collected in the western, central and eastern regions of Croatia during February and March 2015. Aflatoxin M1 (AFM1) concentrations were quantified by the enzyme immunoassay method. The method limits of detection (LOD) and quantification (LOQ) were 22.2 and 34.2 ng/kg, respectively. The mean AFM1 levels measured in the three regions were (ng/kg) as follows: western 3.69, central 3.11 and eastern 4.14. In total, the 548 samples analysed concentrations were below the LOD value and accordingly below the European Union maximum residue level (EU MRL) of 50 ng/kg. The results suggest an absence of use of contaminated with aflatoxin B1 supplementary feedstuff for lactating cows in winter 2015. Such results might be related to the improved storage conditions for feed as well as to the enhanced and more stringent feed control system for mycotoxins in Croatia.
