Assessment of Soil Enzyme Activities in Plant Root Zone of Saline Soil Reclaimed by Drip Irrigation with Saline Groundwater

Abstract

Drip irrigation with saline water is frequently adopted to realize the sustainable utilization of saline–sodic soil with high water tables, and soil enzyme activities can be used to indicate changes in soil quality. In the current study, spatiotemporal changes in soil urease enzyme (URE), alkaline phosphatase (ALP) and invertase (INV) activities were investigated during consecutive growing seasons. Soil in beds was sampled before planting (0 y) and one, two, three and four years after the growing season (1 y, 2 y, 3 y, 4 y), and these samples were distributed at four horizontal distances from the drip line (0, 10, 20 and 30 cm) and four vertical soil depths (0–10, 10–20, 20–30 and 30–40 cm). The results showed that a distribution pattern of URE and ALP activities formed during the first growing season, while the distribution of INV activity formed until the third growing season. All three soil enzyme activities in the upper soil layers and positions close to the drip line were more greatly affected by planting year. The average URE activity of the soil profile decreased slightly during the first year and increased by about 220% and decreased by 20% after reclamation for two and three years, and finally, it increased to 4.9 μg NH4+·g−1·h−1 at the end of the fourth growing season. ALP activity remained stable during the first two years and rapidly increased in the following years; in particular, in the fourth year, it reached 32.7 μg ph(OH)·g−1·h−1. INV activity increased continually with the number of years after planting and reached 1009.0 μg glu·g−1·h−1 at the fourth season’s end. An analysis of variance indicated that URE, ALP and INV activities varied insignificantly among the time points of 0 y, 1 y, 2 y and 3 y (p < 0.05), while they were significantly higher for 4 y than for 0 y and 1 y. In addition, all three enzyme activities of the soil profile had an exponentially increasing trend with the number of years after planting. These results indicated the soil quality in saline–sodic soils could be improved with time under drip irrigation with local saline groundwater, especially around the drip line.

Keywords

• alkaline phosphatase activity (ALP)
• invertase activity (INV)
• planting year
• urease enzyme activity (URE)