LIST: A Newly Developed Laser-assisted Cell Bioprinting Technology
Katiane Roversi,
Hamid Ebrahimi Orimi,
Mahyar Erfanian,
Sebastien Talbot,
Christos Boutopoulos
Affiliations
Katiane Roversi
Département de Pharmacologie et Physiologie, Université de Montréal, Montréal, QC, CanadaCentre de Recherche Hôpital Maisonneuve-Rosemont, Montréal, QC, Canada, Département d'Ophtalmologie, Université de Montréal, Montréal, QC, Canada
Hamid Ebrahimi Orimi
Centre de Recherche Hôpital Maisonneuve-Rosemont, Montréal, QC, CanadaDepartment of Mechanical, Industrial and Aerospace Engineering, Concordia University, Montréal, QC, Canada
Mahyar Erfanian
Centre de Recherche Hôpital Maisonneuve-Rosemont, Montréal, QC, CanadaInstitut de Génie Biomédical, Université de Montréal, Montréal, QC, Canada
Sebastien Talbot
Département de Pharmacologie et Physiologie, Université de Montréal, Montréal, QC, Canada
Christos Boutopoulos
Centre de Recherche Hôpital Maisonneuve-Rosemont, Montréal, QC, CanadaDépartement d'Ophtalmologie, Université de Montréal, Montréal, QC, Canada, Institut de Génie Biomédical, Université de Montréal, Montréal, QC, Canada
Cell bioprinting technologies aim to fabricate tissue-like constructs by delivering biomaterials layer-by-layer. Bioprinted constructs can reduce the use of animals in drug development and hold promise for addressing the shortage of organs for transplants. We recently introduced a laser-assisted drop-on-demand bioprinting technology termed Laser Induced Side Transfer (LIST). This technology can print delicate cell types, including primary neurons. This bioprinting protocol includes the following key steps: cell harvesting, bio-ink preparation, laser setup priming, printing, and post-printing analysis. This protocol includes a detailed description of the laser setup, which is a rather unusual setup for a biology lab. This should allow easy reproduction by readers with basic knowledge of optics. Although we have focused on neuron bioprinting, interested readers will be able to adapt the protocol to bioprint virtually any cell type.Graphical abstract:
