Journal of Experimental & Clinical Cancer Research (Jul 2010)

Expression of miRNA-106b in conventional renal cell carcinoma is a potential marker for prediction of early metastasis after nephrectomy

  • Slaby Ondrej,
  • Jancovicova Jana,
  • Lakomy Radek,
  • Svoboda Marek,
  • Poprach Alexandr,
  • Fabian Pavel,
  • Kren Leos,
  • Michalek Jaroslav,
  • Vyzula Rostislav

DOI
https://doi.org/10.1186/1756-9966-29-90
Journal volume & issue
Vol. 29, no. 1
p. 90

Abstract

Read online

Abstract Background MicroRNAs are endogenously expressed regulatory noncoding RNAs. Previous studies have shown altered expression levels of several microRNAs in renal cell carcinoma. Methods We examined the expression levels of selected microRNAs in 38 samples of conventional renal cell carcinoma (RCC) and 10 samples of non-tumoral renal parenchyma using TaqMan real-time PCR method. Results The expression levels of miRNA-155 (p Conclusions We have confirmed previous observations obtained by miRNA microarray analysis using standardized real-time PCR method. For the first time, we have identified a prognostic significance of miRNA-106b, which, after validation on a larger group of patients, maybe useful as a promising biomarker in patients with RCC.