Identification and characterization of host proteins interacting with NSm, the Tomato spotted wilt virus movement protein

Abstract

To search for host proteins involved in systemic spreading of Tomato spotted wilt virus (TSWV), the virus-encoded NSm movement protein has been utilized as a bait in yeast two-hybrid interaction trap assays. J-domain chaperones from different host species and a protein denominated At-4/1 from Arabidopsis thaliana showing homologies to myosins and kinesins were identified as NSm-interacting partners. In this communication we illustrate that following TSWV infection, J-domain proteins accumulated in systemically infected leaves of A. thaliana, whereas At-4/1 was constitutively detected in leaves of A. thaliana and Nicotiana rustica.

Keywords

• tomato spotted wilt virus (tswv)
• arabidopsis thaliana
• nicotiana rustica
• movement protein
• j-domain protein
• yeast two-hybrid system