Data on the characterization of follicle-stimulating hormone monoclonal antibodies and localization in Japanese eel pituitary
Dae-Jung Kim,
Chae-Won Park,
Munkhzaya Byambaragchaa,
Shin-Kwon Kim,
Bae-Ik Lee,
Hyung-Kyu Hwang,
Jeong-In Myeong,
Sun-Mee Hong,
Myung-Hwa Kang,
Kwan-Sik Min
Affiliations
Dae-Jung Kim
Aquaculture Research Division, National Institute of Fisheries Science (NIFS), Busan 46083, Republic of Korea
Chae-Won Park
Animal Biotechnology, Graduate School of Future Convergence Technology, Institute of Genetic Engineering, Hankyong National University, Anseong 17579, Republic of Korea
Munkhzaya Byambaragchaa
Animal Biotechnology, Graduate School of Future Convergence Technology, Institute of Genetic Engineering, Hankyong National University, Anseong 17579, Republic of Korea
Shin-Kwon Kim
Aquaculture Research Division, National Institute of Fisheries Science (NIFS), Busan 46083, Republic of Korea
Bae-Ik Lee
Aquaculture Research Division, National Institute of Fisheries Science (NIFS), Busan 46083, Republic of Korea
Hyung-Kyu Hwang
Aquaculture Research Division, National Institute of Fisheries Science (NIFS), Busan 46083, Republic of Korea
Jeong-In Myeong
Aquaculture Research Division, National Institute of Fisheries Science (NIFS), Busan 46083, Republic of Korea
Sun-Mee Hong
Dept. of Research and Development, Institute of Gyeongbuk Marine Bioindustry, Ulgin, 36315, Republic of Korea
Myung-Hwa Kang
Department of Food Science & Nutrition, Hoseo University, Asan 31499, Republic of Korea
Kwan-Sik Min
Animal Biotechnology, Graduate School of Future Convergence Technology, Institute of Genetic Engineering, Hankyong National University, Anseong 17579, Republic of Korea; Corresponding author.
Monoclonal antibodies were generated against recombinant follicle-stimulating hormone (rec-FSH) from Japanese eel Anguilla japonica; rec-FSH was produced in Escherichia coli and purified using Ni-NTA Sepharose column chromatography.In support of our recent publication, ''Production and characterization of monoclonal antibodies against recombinant tethered follicle-stimulating hormone from Japanese eel Anguilla japonica'' [1], it was important to characterize the specificity of eel follicle-stimulating hormone antibodies. Here, the production and ELISA system of these monoclonal antibodies are presented. The affinity-purified monoclonal antibodies specifically detected eel rec-FSH in ELISA and on western blots of rec-FSH produced from CHO cells. Immunohistochemical analysis revealed that FSH staining was specifically localized in the eel pituitary. Keywords: Japanese eel, FSH, Monoclonal Antibody
