Nature Communications (Mar 2020)
Amino acid levels determine metabolism and CYP450 function of hepatocytes and hepatoma cell lines
- Ruben Boon,
- Manoj Kumar,
- Tine Tricot,
- Ilaria Elia,
- Laura Ordovas,
- Frank Jacobs,
- Jennifer One,
- Jonathan De Smedt,
- Guy Eelen,
- Matthew Bird,
- Philip Roelandt,
- Ginevra Doglioni,
- Kim Vriens,
- Matteo Rossi,
- Marta Aguirre Vazquez,
- Thomas Vanwelden,
- François Chesnais,
- Adil El Taghdouini,
- Mustapha Najimi,
- Etienne Sokal,
- David Cassiman,
- Jan Snoeys,
- Mario Monshouwer,
- Wei-Shou Hu,
- Christian Lange,
- Peter Carmeliet,
- Sarah-Maria Fendt,
- Catherine M. Verfaillie
Affiliations
- Ruben Boon
- Department of Development and Regeneration, Stem Cell Institute, KU Leuven
- Manoj Kumar
- Department of Development and Regeneration, Stem Cell Institute, KU Leuven
- Tine Tricot
- Department of Development and Regeneration, Stem Cell Institute, KU Leuven
- Ilaria Elia
- Laboratory of Cellular Metabolism and Metabolic Regulation, VIB Center for Cancer Biology, VIB
- Laura Ordovas
- Department of Development and Regeneration, Stem Cell Institute, KU Leuven
- Frank Jacobs
- Janssen Research and Development
- Jennifer One
- Department of Chemical Engineering and Materials Science, University of Minnesota
- Jonathan De Smedt
- Department of Development and Regeneration, Stem Cell Institute, KU Leuven
- Guy Eelen
- Laboratory of Angiogenesis and Vascular Metabolism, Department of Oncology, KU Leuven
- Matthew Bird
- Hepatology, Department of Clinical and Experimental Medicine, KU Leuven
- Philip Roelandt
- Department of Development and Regeneration, Stem Cell Institute, KU Leuven
- Ginevra Doglioni
- Laboratory of Cellular Metabolism and Metabolic Regulation, VIB Center for Cancer Biology, VIB
- Kim Vriens
- Laboratory of Cellular Metabolism and Metabolic Regulation, VIB Center for Cancer Biology, VIB
- Matteo Rossi
- Laboratory of Cellular Metabolism and Metabolic Regulation, VIB Center for Cancer Biology, VIB
- Marta Aguirre Vazquez
- Department of Development and Regeneration, Stem Cell Institute, KU Leuven
- Thomas Vanwelden
- Department of Development and Regeneration, Stem Cell Institute, KU Leuven
- François Chesnais
- Department of Development and Regeneration, Stem Cell Institute, KU Leuven
- Adil El Taghdouini
- Laboratory of Pediatric Hepatology and Cell Therapy, Universit Catholique de Louvain & Cliniques Universitaires St Luc, Institut de Recherche Clinique et Expérimentale (IREC)
- Mustapha Najimi
- Laboratory of Pediatric Hepatology and Cell Therapy, Universit Catholique de Louvain & Cliniques Universitaires St Luc, Institut de Recherche Clinique et Expérimentale (IREC)
- Etienne Sokal
- Laboratory of Pediatric Hepatology and Cell Therapy, Universit Catholique de Louvain & Cliniques Universitaires St Luc, Institut de Recherche Clinique et Expérimentale (IREC)
- David Cassiman
- Hepatology, Department of Clinical and Experimental Medicine, KU Leuven
- Jan Snoeys
- Department of Chemical Engineering and Materials Science, University of Minnesota
- Mario Monshouwer
- Department of Chemical Engineering and Materials Science, University of Minnesota
- Wei-Shou Hu
- Department of Chemical Engineering and Materials Science, University of Minnesota
- Christian Lange
- Laboratory of Angiogenesis and Vascular Metabolism, Department of Oncology, KU Leuven
- Peter Carmeliet
- Laboratory of Angiogenesis and Vascular Metabolism, Department of Oncology, KU Leuven
- Sarah-Maria Fendt
- Laboratory of Cellular Metabolism and Metabolic Regulation, VIB Center for Cancer Biology, VIB
- Catherine M. Verfaillie
- Department of Development and Regeneration, Stem Cell Institute, KU Leuven
- DOI
- https://doi.org/10.1038/s41467-020-15058-6
- Journal volume & issue
-
Vol. 11,
no. 1
pp. 1 – 16
Abstract
Hepatocytes grown in a dish are immature and do not metabolize compounds as a real liver would. Here, the authors supply stem cell-derived hepatocytes with amino acids at a higher concentration than nutritionally necessary, changing the metabolism of these cells, making them more mature and useful for drug screening and toxicity studies.
