Three-dimensional CRISPR screening reveals epigenetic interaction with anti-angiogenic therapy

Michael Y. He; Michael M. Halford; Ruofei Liu; James P. Roy; Zoe L. Grant; Leigh Coultas; Niko Thio; Omer Gilan; Yih-Chih Chan; Mark A. Dawson; Marc G. Achen; Steven A. Stacker

doi:10.1038/s42003-021-02397-3

Communications Biology (Jul 2021)

Three-dimensional CRISPR screening reveals epigenetic interaction with anti-angiogenic therapy

Michael Y. He,
Michael M. Halford,
Ruofei Liu,
James P. Roy,
Zoe L. Grant,
Leigh Coultas,
Niko Thio,
Omer Gilan,
Yih-Chih Chan,
Mark A. Dawson,
Marc G. Achen,
Steven A. Stacker

Affiliations

Michael Y. He: Tumour Angiogenesis and Microenvironment Program, Peter MacCallum Cancer Centre
Michael M. Halford: Tumour Angiogenesis and Microenvironment Program, Peter MacCallum Cancer Centre
Ruofei Liu: Tumour Angiogenesis and Microenvironment Program, Peter MacCallum Cancer Centre
James P. Roy: Tumour Angiogenesis and Microenvironment Program, Peter MacCallum Cancer Centre
Zoe L. Grant: Epigenetics and Development Division, The Walter and Eliza Hall Institute of Medical Research
Leigh Coultas: Epigenetics and Development Division, The Walter and Eliza Hall Institute of Medical Research
Niko Thio: Bioinformatics Core, Peter MacCallum Cancer Centre
Omer Gilan: Sir Peter MacCallum Department of Oncology, The University of Melbourne
Yih-Chih Chan: Translational Haematology Program, Peter MacCallum Cancer Centre
Mark A. Dawson: Sir Peter MacCallum Department of Oncology, The University of Melbourne
Marc G. Achen: Tumour Angiogenesis and Microenvironment Program, Peter MacCallum Cancer Centre
Steven A. Stacker: Tumour Angiogenesis and Microenvironment Program, Peter MacCallum Cancer Centre

DOI: https://doi.org/10.1038/s42003-021-02397-3
Journal volume & issue: Vol. 4, no. 1
pp. 1 – 15

Abstract

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Through three-dimensional CRISPR screening, He et al. report that functional inhibition of BET family of proteins BRD2/3/4 shows mitigating effects on blood endothelial cell (EC) survival and/or proliferation upon VEGFA blockade. An interaction between epigenetic regulation and anti-angiogenesis, which may affect chromosomal structure and activity in ECs through CDC25B phosphatase, is potentially involved with EC resistance to anti-angiogenic therapy.

Published in Communications Biology

ISSN: 2399-3642 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science: Biology (General)
Website: https://www.nature.com/commsbio/

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