Frontiers in Genetics (Oct 2019)
Comprehensive RNA-Seq Data Analysis Identifies Key mRNAs and lncRNAs in Atrial Fibrillation
- Dong-Mei Wu,
- Dong-Mei Wu,
- Zheng-Kun Zhou,
- Shao-Hua Fan,
- Shao-Hua Fan,
- Zi-Hui Zheng,
- Xin Wen,
- Xin Wen,
- Xin-Rui Han,
- Xin-Rui Han,
- Shan Wang,
- Shan Wang,
- Yong-Jian Wang,
- Yong-Jian Wang,
- Zi-Feng Zhang,
- Zi-Feng Zhang,
- Qun Shan,
- Qun Shan,
- Meng-Qiu Li,
- Meng-Qiu Li,
- Bin Hu,
- Bin Hu,
- Jun Lu,
- Jun Lu,
- Gui-Quan Chen,
- Xiao-Wu Hong,
- Yuan-Lin Zheng,
- Yuan-Lin Zheng
Affiliations
- Dong-Mei Wu
- Key Laboratory for Biotechnology on Medicinal Plants of Jiangsu Province, School of Life Science, Jiangsu Normal University, Xuzhou, China
- Dong-Mei Wu
- College of Health Sciences, Jiangsu Normal University, Xuzhou, China
- Zheng-Kun Zhou
- College of Health Sciences, Jiangsu Normal University, Xuzhou, China
- Shao-Hua Fan
- Key Laboratory for Biotechnology on Medicinal Plants of Jiangsu Province, School of Life Science, Jiangsu Normal University, Xuzhou, China
- Shao-Hua Fan
- College of Health Sciences, Jiangsu Normal University, Xuzhou, China
- Zi-Hui Zheng
- State Key Laboratory Cultivation Base For TCM Quality and Efficacy, School of Medicine and Life Science, Nanjing University of Chinese Medicine, Nanjing, China
- Xin Wen
- Key Laboratory for Biotechnology on Medicinal Plants of Jiangsu Province, School of Life Science, Jiangsu Normal University, Xuzhou, China
- Xin Wen
- College of Health Sciences, Jiangsu Normal University, Xuzhou, China
- Xin-Rui Han
- Key Laboratory for Biotechnology on Medicinal Plants of Jiangsu Province, School of Life Science, Jiangsu Normal University, Xuzhou, China
- Xin-Rui Han
- College of Health Sciences, Jiangsu Normal University, Xuzhou, China
- Shan Wang
- Key Laboratory for Biotechnology on Medicinal Plants of Jiangsu Province, School of Life Science, Jiangsu Normal University, Xuzhou, China
- Shan Wang
- College of Health Sciences, Jiangsu Normal University, Xuzhou, China
- Yong-Jian Wang
- Key Laboratory for Biotechnology on Medicinal Plants of Jiangsu Province, School of Life Science, Jiangsu Normal University, Xuzhou, China
- Yong-Jian Wang
- College of Health Sciences, Jiangsu Normal University, Xuzhou, China
- Zi-Feng Zhang
- Key Laboratory for Biotechnology on Medicinal Plants of Jiangsu Province, School of Life Science, Jiangsu Normal University, Xuzhou, China
- Zi-Feng Zhang
- College of Health Sciences, Jiangsu Normal University, Xuzhou, China
- Qun Shan
- Key Laboratory for Biotechnology on Medicinal Plants of Jiangsu Province, School of Life Science, Jiangsu Normal University, Xuzhou, China
- Qun Shan
- College of Health Sciences, Jiangsu Normal University, Xuzhou, China
- Meng-Qiu Li
- Key Laboratory for Biotechnology on Medicinal Plants of Jiangsu Province, School of Life Science, Jiangsu Normal University, Xuzhou, China
- Meng-Qiu Li
- College of Health Sciences, Jiangsu Normal University, Xuzhou, China
- Bin Hu
- Key Laboratory for Biotechnology on Medicinal Plants of Jiangsu Province, School of Life Science, Jiangsu Normal University, Xuzhou, China
- Bin Hu
- College of Health Sciences, Jiangsu Normal University, Xuzhou, China
- Jun Lu
- Key Laboratory for Biotechnology on Medicinal Plants of Jiangsu Province, School of Life Science, Jiangsu Normal University, Xuzhou, China
- Jun Lu
- College of Health Sciences, Jiangsu Normal University, Xuzhou, China
- Gui-Quan Chen
- State Key Laboratory of Pharmaceutical Biotechnology, MOE Key Laboratory of Model Animal for Disease Study, Model Animal Research Center, Nanjing University, Nanjing, China
- Xiao-Wu Hong
- Department of Immunology, School of Basic Medical Sciences, Fudan University, Shanghai, China
- Yuan-Lin Zheng
- Key Laboratory for Biotechnology on Medicinal Plants of Jiangsu Province, School of Life Science, Jiangsu Normal University, Xuzhou, China
- Yuan-Lin Zheng
- College of Health Sciences, Jiangsu Normal University, Xuzhou, China
- DOI
- https://doi.org/10.3389/fgene.2019.00908
- Journal volume & issue
-
Vol. 10
Abstract
Long non-coding RNAs (lncRNAs) are an emerging class of RNA species that may play a critical regulatory role in gene expression. However, the association between lncRNAs and atrial fibrillation (AF) is still not fully understood. In this study, we used RNA sequencing data to identify and quantify the both protein coding genes (PCGs) and lncRNAs. The high enrichment of these up-regulated genes in biological functions concerning response to virus and inflammatory response suggested that chronic viral infection may lead to activated inflammatory pathways, thereby alter the electrophysiology, structure, and autonomic remodeling of the atria. In contrast, the downregulated GO terms were related to the response to saccharides. To identify key lncRNAs involved in AF, we predicted lncRNAs regulating expression of the adjacent PCGs, and characterized biological function of the dysregulated lncRNAs. We found that two lncRNAs, ETF1P2, and AP001053.11, could interact with protein-coding genes (PCGs), which were implicated in AF. In conclusion, we identified key PCGs and lncRNAs, which may be implicated in AF, which not only improves our understanding of the roles of lncRNAs in AF, but also provides potentially functional lncRNAs for AF researchers.
Keywords
