AtTrxh3, a Thioredoxin, Is Identified as an Abscisic AcidBinding Protein in <i>Arabidopsis thaliana</i>
Tomoaki Anabuki,
Keisuke Ohashi,
Taichi E. Takasuka,
Hideyuki Matsuura,
Kosaku Takahashi
Affiliations
Tomoaki Anabuki
Division of Fundamental Agriscience Research, Research Faculty of Agriculture, Hokkaido University, Kita 9 Nishi 9, Kita-ku, Sapporo 060-8589, Japan
Keisuke Ohashi
Graduate School of Global Food Resources, Hokkaido University, Kita 9 Nishi 9, Kita-ku, Sapporo 060-0809, Japan
Taichi E. Takasuka
Division of Fundamental Agriscience Research, Research Faculty of Agriculture, Hokkaido University, Kita 9 Nishi 9, Kita-ku, Sapporo 060-8589, Japan
Hideyuki Matsuura
Division of Fundamental Agriscience Research, Research Faculty of Agriculture, Hokkaido University, Kita 9 Nishi 9, Kita-ku, Sapporo 060-8589, Japan
Kosaku Takahashi
Department of Nutritional Science, Faculty of Applied Bioscience, Tokyo University of Agriculture, 1-1-1 Sakuragaoka, Setagaya-ku, Tokyo 165-8502, Japan
Abscisic acid (ABA, 1) is a plant hormone that regulates various plant physiological processes such as seed developing and stress responses. The ABA signaling system has been elucidated; binding of ABA with PYL proteins triggers ABA signaling. We have previously reported a new method to isolate a protein targeted with a bioactive small molecule using a biotin linker with alkyne and amino groups, a protein cross-linker, and a bioactive small molecule with an azido group (azido probe). This method was used to identify the unknown ABA binding protein of Arabidopsis thaliana. As a result, AtTrxh3, a thioredoxin, was isolated as an ABA binding protein. Our developed method can be applied to the identification of binding proteins of bioactive compounds.
