STAR Protocols (Dec 2021)

Global Run-On sequencing to measure nascent transcription in C. elegans

  • Piergiuseppe Quarato,
  • Germano Cecere

Journal volume & issue
Vol. 2, no. 4
p. 100991

Abstract

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Summary: Global Run-On sequencing (GRO-seq) is one of the most sensitive techniques to detect nascent transcription from RNA polymerase (Pol) at a genome-wide level. The protocol incorporates labeled ribonucleotides into nascent RNAs from Pol I, II, and III. We have adapted the GRO-seq protocol to the nematode Caenorhabditis elegans to measure transcription from embryos and adult worms. Here, we provide a detailed overview of the protocol highlighting the critical steps for generating successful libraries.For complete details on the use and execution of this protocol, please refer to Quarato et al. (2021).

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