STAR Protocols (Dec 2021)
Global Run-On sequencing to measure nascent transcription in C. elegans
Abstract
Summary: Global Run-On sequencing (GRO-seq) is one of the most sensitive techniques to detect nascent transcription from RNA polymerase (Pol) at a genome-wide level. The protocol incorporates labeled ribonucleotides into nascent RNAs from Pol I, II, and III. We have adapted the GRO-seq protocol to the nematode Caenorhabditis elegans to measure transcription from embryos and adult worms. Here, we provide a detailed overview of the protocol highlighting the critical steps for generating successful libraries.For complete details on the use and execution of this protocol, please refer to Quarato et al. (2021).