The differences in cell wall appearance in sensitive and resistant Staphylococcus aureus to the antibiotic vancomycin from diabetic ulcer isolates in lampung province indonesia using transmission microscope electron

Abstract

AIM: This study aims to visually ascertain the differences in cell walls in Staphylococcus aureus which is sensitive and resistant to Vancomycin. Background. S. aureus is a common cause of infections that occur in hospitals. Over the past few decades, there has been an alarming increase in the prevalence of pathogens and strains resistant of S. aureus to antibiotics including Vancomycin. Resistance to vancomycin is carried by the vanA gene, characterized by thickening of the cell walls of S. aureus bacteria. METHODS: The research sample consisted of 19 isolates of S. aureus bacteria taken from diabetic ulcer swabs which were then subjected to the Minimum Inhibitory Concentration (MIC) test and visual examination of cell wall thickness by Transmission Microscope Electron (TEM). Result. The results of the study showed that 2 samples were Vancomycin-resistant Staphylococcus aureus (VRSA) based on the MIC test for the antibiotic Vancomycin with a MIC value of 32 μg/ML, which indicates that there was a strain that was resistant to the antibiotic Vancomycin because it had a MIC value more than of 16 μg/ML. There is a difference in the cell wall thickness of Vancomycin-sensitive Staphylococcus aureus (VSSA) and VRSA as optically discerned by the TEM. Based on the Independent T-test, observation of the cell walls of VSSA and VRSA bacteria showed a significant difference in thickness. CONCLUSION: This study concludes that there is a difference of the cell wall thickness of Vancomycin-sensitive Staphylococcus aureus and Vancomycin-resistant Staphylococcus aureus as optically discerned by the TEM.

Keywords

• Cell wall
• VRSA
• TEM