Bio-Protocol
(Feb 2014)
Intracellular Staining for Phosphorylated STAT4 and STAT5 in Mouse Splenocytes
Ana Villegas-Mendez,
J. de Souza,
Seen-Wai Lavelle,
Emily Findlay,
Tovah Shaw,
Christiaan Saris,
Christopher Hunter,
Eleanor Riley ,
Kevin Couper
Affiliations
Ana Villegas-Mendez
Department of Immunology and Infection, Faculty of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, London, UK
J. de Souza
Department of Immunology and Infection, Faculty of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, London, UK
Seen-Wai Lavelle
Department of Immunology and Infection, Faculty of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, London, UK
Emily Findlay
Department of Immunology and Infection, Faculty of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, London, UK
Tovah Shaw
Department of Immunology and Infection, Faculty of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, London, UK
Christiaan Saris
Department of Inflammation Research, Amgen, Inc., Thousand Oaks, CA, USA
Christopher Hunter
Department of Pathobiology, University of Pennsylvania, Philadelphia, USA
Eleanor Riley
Department of Immunology and Infection, Faculty of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, London, UK
Kevin Couper
Department of Immunology and Infection, Faculty of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, London, UK
DOI
https://doi.org/10.21769/BioProtoc.1043
Journal volume & issue
Vol. 4,
no. 3
Abstract
Read online
The Stat (Signal Transducer and Activator of Transcription) family of proteins are critical signal transducers involved in fundamental cellular processes, including cell growth and differentiation, development, apoptosis, immune responses and inflammation. In here, we describe a simple and reproducible flow cytometry protocol to measure Stat protein phosphorylation in splenocyte preparations from malaria infected mice.
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