Optical characterization of the liver tissue affected by fibrolamellar hepatocellular carcinoma based on internal filters of laser-induced fluorescence

Abstract

Abstract Laser-induced fluorescence (LIF) spectroscopy has recently gained regards for diagnosis of the cancer in various tissues of the human body. This method in its conventional form, when used for assay of highly scattering media, encounters a lot of noise due to multiple scattering and inner filter effects which overshadows the sensitivity and specificity of the method. Here, angular dependence of the LIF spectral shift due to the reabsorption events have been investigated for characterization of the bio-tissues. The aim was to determine the tissue morphological changeovers due to the cancer progression. The assessment of a rare type of the liver cancer i. e. fibrolamellar hepatocellular carcinoma revealed the significant difference in optical anisotropy of the parenchyma and liver tumor. As a result, utilizing LIF spectroscopy as a fast, highly sensitive and easy-to-use method one can evaluate the optical anisotropy for diagnosing tissues during the cancer progression.