Investigations into the fire resistance of high-strength concrete (HSC) is extremely important to optimize structural design in construction engineering. This work describes the influence of polypropylene fibers on the mechanical properties and durability of HSC at high temperatures (25, 100, 200, 400, 600 and 800 °C). HSC specimens with 2 kg/m3 composed of polypropylene fibers are tested in a temperature range of 25 to 800 °C, followed by microstructural analysis. In addition, a statistical analysis is designed to identify the effect of factors, namely temperature and polypropylene fibers, and their interactions on mechanical properties and water absorption, electrical resistivity, mass loss and ultrasonic velocity. Most of the properties are improved by the incorporation of fibers, obtaining highly predictable regression models. However, the polypropylene fibers reduce compressive strength but improve the residual mechanical properties up to 400 °C.