Luminol–K3Fe(CN)6 chemiluminescence system for the determination of glipizide

Abstract

A rapid and sensitive flow-injection chemiluminescence (CL) method for the determination of glipizide was developed on the basis of finding that glipizide can enhance the CL intensity of the luminol–K3Fe(CN)6 system. In optimum condition, the increased CL intensity was directly proportional to the concentration of glipizide in the range from 4.0×10−8 g/mL to 1.0×10−6 g/mL and the detection limit was 1.0×10−8 g/mL glipizide. The relative standard deviation (RSD) of the developed method was 2.1% with 11 repeated measurements of 1.0×10−7 g/mL glipizide. The developed method has been successfully applied to the analysis of glipizide in its pharmaceutical preparations. Keywords: Luminol–K3Fe(CN)6, Glipizide, Flow injection, Chemiluminescence