Sporadic Parathyroid Adenoma: A Pilot Study of Novel Biomarkers in Females
Angeliki Cheva,
Angeliki Chorti,
Kassiani Boulogeorgou,
Anthoula Chatzikyriakidou,
Charoula Achilla,
Vangelis Bontinis,
Alkis Bontinis,
Stefanos Milias,
Thomas Zarampoukas,
Sohail Y. Bakkar,
Theodosios Papavramidis
Affiliations
Angeliki Cheva
Laboratory of Pathology, Faculty of Health Science, Medical School, Aristotle University, 541 24 Thessaloniki, Greece
Angeliki Chorti
1st Propaedeutic Department of Surgery, Faculty of Health Science, Medical School, AHEPA University Hospital, Aristotle University, 546 36 Thessaloniki, Greece
Kassiani Boulogeorgou
Laboratory of Pathology, Faculty of Health Science, Medical School, Aristotle University, 541 24 Thessaloniki, Greece
Anthoula Chatzikyriakidou
Laboratory of Medical Biology—Genetics, Faculty of Health Science, Medical School, Aristotle University, 546 36 Thessaloniki, Greece
Charoula Achilla
Laboratory of Medical Biology—Genetics, Faculty of Health Science, Medical School, Aristotle University, 546 36 Thessaloniki, Greece
Vangelis Bontinis
Department of Vascular Surgery, Faculty of Health Science, Medical School, AHEPA University Hospital, Aristotle University, 546 36 Thessaloniki, Greece
Alkis Bontinis
Department of Vascular Surgery, Faculty of Health Science, Medical School, AHEPA University Hospital, Aristotle University, 546 36 Thessaloniki, Greece
Laboratory of Pathology, Interbalkan Medical Center, 546 26 Thessaloniki, Greece
Sohail Y. Bakkar
Endocrine & General Surgery, The Hashemite University, Amman 13133, Jordan
Theodosios Papavramidis
1st Propaedeutic Department of Surgery, Faculty of Health Science, Medical School, AHEPA University Hospital, Aristotle University, 546 36 Thessaloniki, Greece
Background and Objectives: Parathyroid adenoma is a distinct cause of primary hyperparathyroidism, with the vast majority being sporadic ones. Proteomic analysis of parathyroid adenomas has proposed a large number of related proteins. The aim of this study is to evaluate the immunohistochemical staining of ANXA2, MED12, MAPK1 and VDR in parathyroid adenoma tissue. Materials and Methods: Fifty-one parathyroid adenomas were analyzed for ANXA2, MED12, MAPK1 and VDR expressions. Tissue was extracted from formalin-fixed paraffin-embedded parathyroid adenoma specimens; an immunohistochemical study was applied, and the percentage of allocation and intensity were evaluated. Results: ANXA2 stained positively in 60.8% of all cell types, while MED12 had positive staining in 66%. MAPK1 expression was found to be negative in total, although a specific pattern for oxyphil cells was observed, as they stained positive in 17.7%. Finally, VDR staining was positive at 22.8%, based on nuclear staining. Conclusions: These immunohistochemical results could be utilized as biomarkers for the diagnosis of sporadic parathyroid adenoma. It is of great importance that a distinct immunophenotype of nodule-forming cells in a positive adenoma could suggest a specific pattern of adenoma development, as in hereditary patterns.
