Control of type III protein secretion using a minimal genetic system

Miryoung Song; David J. Sukovich; Luciano Ciccarelli; Julia Mayr; Jesus Fernandez-Rodriguez; Ethan A. Mirsky; Alex C. Tucker; D. Benjamin Gordon; Thomas C. Marlovits; Christopher A. Voigt

doi:10.1038/ncomms14737

Nature Communications (May 2017)

Control of type III protein secretion using a minimal genetic system

Miryoung Song,
David J. Sukovich,
Luciano Ciccarelli,
Julia Mayr,
Jesus Fernandez-Rodriguez,
Ethan A. Mirsky,
Alex C. Tucker,
D. Benjamin Gordon,
Thomas C. Marlovits,
Christopher A. Voigt

Affiliations

Miryoung Song: Department of Biological Engineering, Massachusetts Institute of Technology, Synthetic Biology Center
David J. Sukovich: Department of Biological Engineering, Massachusetts Institute of Technology, Synthetic Biology Center
Luciano Ciccarelli: Center for Structural Systems Biology (CSSB), University Medical Center Hamburg-Eppendorf (UKE)
Julia Mayr: Center for Structural Systems Biology (CSSB), University Medical Center Hamburg-Eppendorf (UKE)
Jesus Fernandez-Rodriguez: Department of Biological Engineering, Massachusetts Institute of Technology, Synthetic Biology Center
Ethan A. Mirsky: Department of Biological Engineering, Massachusetts Institute of Technology, Synthetic Biology Center
Alex C. Tucker: Department of Biological Engineering, Massachusetts Institute of Technology, Synthetic Biology Center
D. Benjamin Gordon: MIT-Broad Foundry, Broad Institute of MIT and Harvard
Thomas C. Marlovits: Center for Structural Systems Biology (CSSB), University Medical Center Hamburg-Eppendorf (UKE)
Christopher A. Voigt: Department of Biological Engineering, Massachusetts Institute of Technology, Synthetic Biology Center

DOI: https://doi.org/10.1038/ncomms14737
Journal volume & issue: Vol. 8, no. 1
pp. 1 – 9

Abstract

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The type III secretion system is a needle-like molecular machine under tight regulatory control. Here the authors construct a synthetic type III secretion system gene cluster by deconstructing and rebuilding the wild-typeSalmonellapathogenicity island 1.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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