Effect of Phosphoric Amide Herbicide APM on the Structure and Protein Composition of Chromosome in Triticum durum

Abstract

The cytological and biochemical effects of APM on the mitotic cells of root meristems of Triticum durumwere studied by exposure to concentrations of 2 fxM to 10 juM for 16 h. The results showed that multipolar spindle cells, chromosome-condensation, bridge-fragments and micronuclei were induced in the cells of rootmeristems by treatment with i/zM APM. Five protein species which have a molecular weight and pi of 40 KD/pI 5.5, 40 KD/pI 5.6, 48 KD/pI 7.0, 88 KD/pI 6.4, 120 KD/pI 6.6 were lost and 12 new protein species which have a molecular weight and pi of 16 KD/pI 6.2, 18 KD/pI 6.7, 19 KD/pI 7.0, 20 KD/pI 6.5, 22 KD/pI 7.0, 23 KD/pI 6.7, 24 KD/pI 4.7, 24 KD/pI 5.8, 26 KD/pI 5.8, 88 KD/pI 6.6, 86 KD/pI 6.7, 95 KD/pI 6.5 were induced by 10 MMAPM in the root meristems.

Keywords

• Amiprophos methyl (APM )
• Chromosome- condensation
• Multipolar division
• 2D SDS-PAGE
• Triticum durum