Guangdong nongye kexue (Apr 2022)
Effects of Host Switching on Diversity of Intestinal Bacteria in Batocera lineolata Larvae
Abstract
【Objective】The study was carried out to investigate the effect of host switching on the diversity of intestinal bacteria in Batocera lineolata larvae, and find out the host adaptability of B. lineolata larvae from the perspective of intestinal bacteria, with a view to providing a theoretical basis for the development and utilization of microbes.【Method】B. lineolata larvae were raised with walnut as primary hosts and Eucalyptus as switching hosts, and the diversity of intestinal bacteria in B. lineolata larvae was studied by using the methods of traditional microbial culture, 16Sr RNA gene sequencing and high-throughput sequencing.【Result】A total of 19 culturable strains of bacteria were obtained through traditional microbial culture. 10 strains of bacteria were isolated from the intestine of the larvae of B. lineolata reared on the original host, and 9 strains were isolated from the larvae of B. reared by host; 6 genera of culturable bacteria were isolated from both treatments, 5 of which were common genera. High-throughput sequencing results showed that there were no significant differences in the diversity of intestinal bacteria in the larvae before and after host switching. B. lineolata larvae intestinal bacteria samples respectively belonged to 20 phylums, 32 classes, 55 orders, 90 families and 170 genera, including, under two treatments, both B.lineolata larvae gut dominant fungi belonging to the Proteobacteri, Gammaproteobacteria, Enterobacteriales, Enterobacteriaceae and Uncultured bacterium f Enterobacteriaceae.【Conclusion】The composition of intestinal bacteria species of B. lineolata larvae under two treatments was similar, the overall diversity of intestinal bacterial community was relatively rich and stable, and it was difficult to change the diversity of intestinal bacteria by changing the host in a short period of time.
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