The adipose tissue of origin influences the biological potential of human adipose stromal cells isolated from mediastinal and subcutaneous fat depots

Camilla Siciliano; Antonella Bordin; Mohsen Ibrahim; Isotta Chimenti; Francesco Cassiano; Ilenia Gatto; Giorgio Mangino; Andrea Coccia; Selenia Miglietta; Daniela Bastianelli; Vincenzo Petrozza; Antonella Calogero; Giacomo Frati; Elena De Falco

doi:10.1016/j.scr.2016.07.010

Stem Cell Research (Sep 2016)

The adipose tissue of origin influences the biological potential of human adipose stromal cells isolated from mediastinal and subcutaneous fat depots

Camilla Siciliano,
Antonella Bordin,
Mohsen Ibrahim,
Isotta Chimenti,
Francesco Cassiano,
Ilenia Gatto,
Giorgio Mangino,
Andrea Coccia,
Selenia Miglietta,
Daniela Bastianelli,
Vincenzo Petrozza,
Antonella Calogero,
Giacomo Frati,
Elena De Falco

Affiliations

Camilla Siciliano: Department of Medical-Surgical Sciences and Biotechnologies, Sapienza University of Rome Polo Pontino, C.so della Repubblica 79, 04100 Latina, Italy
Antonella Bordin: Department of Medical-Surgical Sciences and Biotechnologies, Sapienza University of Rome Polo Pontino, C.so della Repubblica 79, 04100 Latina, Italy
Mohsen Ibrahim: Division of Thoracic Surgery, Department of Medical-Surgical Science and Translational Medicine, Sapienza University of Rome, S. Andrea Hospital, via di Grottarossa 1035, 00189 Rome, Italy
Isotta Chimenti: Department of Medical-Surgical Sciences and Biotechnologies, Sapienza University of Rome Polo Pontino, C.so della Repubblica 79, 04100 Latina, Italy
Francesco Cassiano: Department of Anatomy, Histology, Forensic Medicine and Orthopaedics, Section of Anatomy, Electron Microscopy Unit, Laboratory “Pietro M. Motta”, Sapienza University of Rome, Rome, Italy
Ilenia Gatto: Department of Medical-Surgical Sciences and Biotechnologies, Sapienza University of Rome Polo Pontino, C.so della Repubblica 79, 04100 Latina, Italy
Giorgio Mangino: Department of Medical-Surgical Sciences and Biotechnologies, Sapienza University of Rome Polo Pontino, C.so della Repubblica 79, 04100 Latina, Italy
Andrea Coccia: Department of Medical-Surgical Sciences and Biotechnologies, Sapienza University of Rome Polo Pontino, C.so della Repubblica 79, 04100 Latina, Italy
Selenia Miglietta: Department of Anatomy, Histology, Forensic Medicine and Orthopaedics, Section of Anatomy, Electron Microscopy Unit, Laboratory “Pietro M. Motta”, Sapienza University of Rome, Rome, Italy
Daniela Bastianelli: Department of Medical-Surgical Sciences and Biotechnologies, Sapienza University of Rome Polo Pontino, C.so della Repubblica 79, 04100 Latina, Italy
Vincenzo Petrozza: Department of Medical-Surgical Sciences and Biotechnologies, Sapienza University of Rome Polo Pontino, C.so della Repubblica 79, 04100 Latina, Italy
Antonella Calogero: Department of Medical-Surgical Sciences and Biotechnologies, Sapienza University of Rome Polo Pontino, C.so della Repubblica 79, 04100 Latina, Italy
Giacomo Frati: Department of Medical-Surgical Sciences and Biotechnologies, Sapienza University of Rome Polo Pontino, C.so della Repubblica 79, 04100 Latina, Italy
Elena De Falco: Department of Medical-Surgical Sciences and Biotechnologies, Sapienza University of Rome Polo Pontino, C.so della Repubblica 79, 04100 Latina, Italy

DOI: https://doi.org/10.1016/j.scr.2016.07.010
Journal volume & issue: Vol. 17, no. 2
pp. 342 – 351

Abstract

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Indirect evidence suggests that adipose tissue-derived stromal cells (ASCs) possess different physiological and biological variations related to the anatomical localization of the adipose depots. Accordingly, to investigate the influence of the tissue origin on the intrinsic properties of ASCs and to assess their response to specific stimuli, we compared the biological, functional and ultrastructural properties of two ASC pools derived from mediastinal and subcutaneous depots (thoracic compartment) by means of supplements such as platelet lysate (PL) and FBS. Subcutaneous ASCs exhibited higher proliferative and clonogenic abilities than mediastinal counterpart, as well as increased secreted levels of IL-6 combined with lower amount of VEGF-C. In contrast, mediastinal ASCs displayed enhanced pro-angiogenic and adipogenic differentiation properties, increased cell diameter and early autophagic processes, highlighted by electron microscopy. Our results further support the hypothesis that the origin of adipose tissue significantly defines the biological properties of ASCs, and that a homogeneric function for all ASCs cannot be assumed.

Published in Stem Cell Research

ISSN: 1873-5061 (Print); 1876-7753 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Science: Biology (General)
Website: https://www.journals.elsevier.com/stem-cell-research

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