PLoS ONE (Aug 2010)

Microarray-based approach identifies differentially expressed microRNAs in porcine sexually immature and mature testes.

  • Lifan Luo,
  • Lianzhi Ye,
  • Gang Liu,
  • Guochao Shao,
  • Rong Zheng,
  • Zhuqing Ren,
  • Bo Zuo,
  • Dequan Xu,
  • Minggang Lei,
  • Siwen Jiang,
  • Changyan Deng,
  • Yuanzhu Xiong,
  • Fenge Li

DOI
https://doi.org/10.1371/journal.pone.0011744
Journal volume & issue
Vol. 5, no. 8
p. e11744

Abstract

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BackgroundMicroRNAs (miRNAs) are short non-coding RNA molecules which are proved to be involved in mammalian spermatogenesis. Their expression and function in the porcine germ cells are not fully understood.MethodologyWe employed a miRNA microarray containing 1260 unique miRNA probes to evaluate the miRNA expression patterns between sexually immature (60-day) and mature (180-day) pig testes. One hundred and twenty nine miRNAs representing 164 reporter miRNAs were expressed differently (pConclusionsOverall, the results of this study indicated specific miRNAs expression in porcine testes and suggested that miRNAs had a role in regulating spermatogenesis.