Protocol for computationally evaluating the loss of stoichiometry and coordinated expression of proteins

Abstract

Summary: Dysregulation of the transcriptional or translational machinery can alter the stoichiometry of multiprotein complexes and occurs in natural processes such as aging. Loss of stoichiometry has been shown to alter protein complex functions. We provide a protocol and associated code that use omics data to quantify these stoichiometric changes via statistical dispersion utilizing the interquartile range of expression values per grouping variable. This descriptive statistical approach enables the quantification of stoichiometry changes without additional data acquisition.For complete details on the use and execution of this protocol, please refer to Hinz et al. (2021).

Keywords

• Bioinformatics
• RNAseq
• Proteomics
• Systems biology