Тонкие химические технологии (Apr 2012)

Elaboration of indirect method (test) for determination of enantiomeric purity of chiral PANAM (polyamide nucleic acids mimetics) monomers

  • Yu. G. Kirillova,
  • A. V. Ryakin,
  • D. I. Prokhorov,
  • M. A. Lyanov,
  • O. V. Esipova,
  • S. V. Eremin,
  • E. E. Zvonkova

Journal volume & issue
Vol. 7, no. 2
pp. 70 – 74


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An indirect approach for the determination of enantiomeric purity of chiral α-(benzyl)-carboxyethyl- and γ-methyl-substituted monomers of polyamide nucleic acids mimics (PANAM) is described. The test consist in the synthesis of C-terminal diastereomers with methyl ester of L- or DL-isoleucine for each PANAM monomer and estimation of the relative content (abundance ratio) of main and minor diastereomers by means of reverse phase HPLC and 1H-NMR spectroscopy.