Biochar-extracted liquor stimulates nitrogen related gene expression on improving nitrogen utilization in rice seedling

Jian Gao; Jian Gao; Shaohua Ge; Shaohua Ge; Hailong Wang; Yunying Fang; Luming Sun; Luming Sun; Tianyi He; Tianyi He; Xiaoyi Cheng; Xiaoyi Cheng; Di Wang; Di Wang; Xuanwei Zhou; Xuanwei Zhou; Heqing Cai; Caibin Li; Yanxiang Liu; Yang E; Yang E; Jun Meng; Jun Meng; Wenfu Chen; Wenfu Chen

doi:10.3389/fpls.2023.1131937

Frontiers in Plant Science (Jun 2023)

Biochar-extracted liquor stimulates nitrogen related gene expression on improving nitrogen utilization in rice seedling

Jian Gao,
Jian Gao,
Shaohua Ge,
Shaohua Ge,
Hailong Wang,
Yunying Fang,
Luming Sun,
Luming Sun,
Tianyi He,
Tianyi He,
Xiaoyi Cheng,
Xiaoyi Cheng,
Di Wang,
Di Wang,
Xuanwei Zhou,
Xuanwei Zhou,
Heqing Cai,
Caibin Li,
Yanxiang Liu,
Yang E,
Yang E,
Jun Meng,
Jun Meng,
Wenfu Chen,
Wenfu Chen

Affiliations

Jian Gao: National Biochar Institute of Shenyang Agricultural University, Shenyang, China
Jian Gao: Key Laboratory of Biochar and Soil Improvement, Ministry of Agriculture and Rural Affairs, Shenyang, China
Shaohua Ge: National Biochar Institute of Shenyang Agricultural University, Shenyang, China
Shaohua Ge: Key Laboratory of Biochar and Soil Improvement, Ministry of Agriculture and Rural Affairs, Shenyang, China
Hailong Wang: School of Environmental and Chemical Engineering, Foshan University, Foshan, China
Yunying Fang: Australian Rivers Institute, School of Environment and Science, Griffith University, Nathan, QLD, Australia
Luming Sun: National Biochar Institute of Shenyang Agricultural University, Shenyang, China
Luming Sun: Key Laboratory of Biochar and Soil Improvement, Ministry of Agriculture and Rural Affairs, Shenyang, China
Tianyi He: National Biochar Institute of Shenyang Agricultural University, Shenyang, China
Tianyi He: Key Laboratory of Biochar and Soil Improvement, Ministry of Agriculture and Rural Affairs, Shenyang, China
Xiaoyi Cheng: National Biochar Institute of Shenyang Agricultural University, Shenyang, China
Xiaoyi Cheng: Key Laboratory of Biochar and Soil Improvement, Ministry of Agriculture and Rural Affairs, Shenyang, China
Di Wang: National Biochar Institute of Shenyang Agricultural University, Shenyang, China
Di Wang: Key Laboratory of Biochar and Soil Improvement, Ministry of Agriculture and Rural Affairs, Shenyang, China
Xuanwei Zhou: National Biochar Institute of Shenyang Agricultural University, Shenyang, China
Xuanwei Zhou: Key Laboratory of Biochar and Soil Improvement, Ministry of Agriculture and Rural Affairs, Shenyang, China
Heqing Cai: Bijie Tobacco Company of Guizhou Province, Bijie, China
Caibin Li: Bijie Tobacco Company of Guizhou Province, Bijie, China
Yanxiang Liu: Bijie Tobacco Company of Guizhou Province, Bijie, China
Yang E: National Biochar Institute of Shenyang Agricultural University, Shenyang, China
Yang E: Key Laboratory of Biochar and Soil Improvement, Ministry of Agriculture and Rural Affairs, Shenyang, China
Jun Meng: National Biochar Institute of Shenyang Agricultural University, Shenyang, China
Jun Meng: Key Laboratory of Biochar and Soil Improvement, Ministry of Agriculture and Rural Affairs, Shenyang, China
Wenfu Chen: National Biochar Institute of Shenyang Agricultural University, Shenyang, China
Wenfu Chen: Key Laboratory of Biochar and Soil Improvement, Ministry of Agriculture and Rural Affairs, Shenyang, China

DOI: https://doi.org/10.3389/fpls.2023.1131937
Journal volume & issue: Vol. 14

Abstract

Read online

IntroductionBiochar has been shown to be an effective soil amendment for promoting plant growth and improving nitrogen (N) utilization. However, the physiological and molecular mechanisms behind such stimulation remain unclear.MethodsIn this study, we investigated whether biochar-extracted liquor including 21 organic molecules enhance the nitrogen use efficiency (NUE) of rice plants using two N forms (NH4+-N and NO3--N). A hydroponic experiment was conducted, and biochar-extracted liquor (between 1 and 3% by weight) was applied to rice seedlings.ResultsThe results showed that biochar-extracted liquor significantly improved phenotypic and physiological traits of rice seedlings. Biochar-extracted liquor dramatically upregulated the expression of rice N metabolism-related genes such as OsAMT1.1, OsGS1.1, and OsGS2. Rice seedlings preferentially absorbed NH4+-N than NO3--N (p < 0.05), and the uptake of NH4+-N by rice seedlings was significantly increased by 33.60% under the treatment of biochar-extracted liquor. The results from molecular docking showed that OsAMT1.1protein can theoretically interact with 2-Acetyl-5-methylfuran, trans-2,4-Dimethylthiane, S, S-dioxide, 2,2-Diethylacetamide, and 1,2-Dimethylaziridine in the biochar-extracted liquor. These four organic compounds have similar biological function as the OsAMT1.1 protein ligand in driving NH4+-N uptakes by rice plants.DiscussionThis study highlights the importance of biochar-extracted liquor in promoting plant growth and NUE. The use of low doses of biochar-extracted liquor could be an important way to reduce N input in order to achieve the purpose of reducing fertilizer use and increasing efficiency in agricultural production.

Published in Frontiers in Plant Science

ISSN: 1664-462X (Online)
Publisher: Frontiers Media S.A.
Country of publisher: Switzerland
LCC subjects: Agriculture: Plant culture
Website: https://www.frontiersin.org/journals/plant-science

About the journal

Abstract

Keywords