Biofabrication of Zinc Oxide Nanoparticles With Syzygium aromaticum Flower Buds Extract and Finding Its Novel Application in Controlling the Growth and Mycotoxins of Fusarium graminearum

Thimappa Ramachandrappa Lakshmeesha; Naveen Kumar Kalagatur; Naveen Kumar Kalagatur; Venkataramana Mudili; Chakrabhavi Dhananjaya Mohan; Shobith Rangappa; Bangari Daruka Prasad; Bagepalli Shivaram Ashwini; Abeer Hashem; Abeer Hashem; Abdulaziz A. Alqarawi; Jahangir Ahmad Malik; Elsayed Fathi Abd_Allah; Vijai Kumar Gupta; Chandra Nayaka Siddaiah; Siddapura Ramachandrappa Niranjana

doi:10.3389/fmicb.2019.01244

Frontiers in Microbiology (Jun 2019)

Biofabrication of Zinc Oxide Nanoparticles With Syzygium aromaticum Flower Buds Extract and Finding Its Novel Application in Controlling the Growth and Mycotoxins of Fusarium graminearum

Thimappa Ramachandrappa Lakshmeesha,
Naveen Kumar Kalagatur,
Naveen Kumar Kalagatur,
Venkataramana Mudili,
Chakrabhavi Dhananjaya Mohan,
Shobith Rangappa,
Bangari Daruka Prasad,
Bagepalli Shivaram Ashwini,
Abeer Hashem,
Abeer Hashem,
Abdulaziz A. Alqarawi,
Jahangir Ahmad Malik,
Elsayed Fathi Abd_Allah,
Vijai Kumar Gupta,
Chandra Nayaka Siddaiah,
Siddapura Ramachandrappa Niranjana

Affiliations

Thimappa Ramachandrappa Lakshmeesha: Department of Studies in Biotechnology, University of Mysore, Mysore, India
Naveen Kumar Kalagatur: Microbiology Division, Defence Food Research Laboratory, Mysore, India
Naveen Kumar Kalagatur: Toxicology and Immunology Division, DRDO-BU-Centre for Life Sciences, Bharathiar University, Coimbatore, India
Venkataramana Mudili: Toxicology and Immunology Division, DRDO-BU-Centre for Life Sciences, Bharathiar University, Coimbatore, India
Chakrabhavi Dhananjaya Mohan: Department of Studies in Molecular Biology, University of Mysore, Mysore, India
Shobith Rangappa: Adichunchanagiri Institute for Molecular Medicine, Mandya, India
Bangari Daruka Prasad: Department of Physics, BMS Institute of Technology and Management, Bengaluru, India
Bagepalli Shivaram Ashwini: East Point College of Medical Sciences and Research Centre, Bengaluru, India
Abeer Hashem: Botany and Microbiology Department, College of Science, King Saud University, Riyadh, Saudi Arabia
Abeer Hashem: Mycology and Plant Disease Survey Department, Plant Pathology Research Institute, Agriculture Research Center (ARC), Giza, Egypt
Abdulaziz A. Alqarawi: 0Department of Plant Production, College of Food and Agricultural Sciences, King Saud University, Riyadh, Saudi Arabia
Jahangir Ahmad Malik: 0Department of Plant Production, College of Food and Agricultural Sciences, King Saud University, Riyadh, Saudi Arabia
Elsayed Fathi Abd_Allah: 0Department of Plant Production, College of Food and Agricultural Sciences, King Saud University, Riyadh, Saudi Arabia
Vijai Kumar Gupta: 1Department of Chemistry and Biotechnology, Tallinn University of Technology, Tallinn, Estonia
Chandra Nayaka Siddaiah: Department of Studies in Biotechnology, University of Mysore, Mysore, India
Siddapura Ramachandrappa Niranjana: Department of Studies in Biotechnology, University of Mysore, Mysore, India

DOI: https://doi.org/10.3389/fmicb.2019.01244
Journal volume & issue: Vol. 10

Abstract

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Fusarium graminearum is a leading plant pathogen that causes Fusarium head blight, stalk rot, and Gibberella ear rot diseases in cereals and posing the immense threat to the microbiological safety of the food. Herein, we report the green synthesis of zinc oxide nanoparticles from Syzygium aromaticum (SaZnO NPs) flower bud extract by combustion method and investigated their application for controlling of growth and mycotoxins of F. graminearum. Formation of SaZnO NPs was confirmed by spectroscopic methods. The electron microscopic (SEM and TEM) analysis revealed the formation of triangular and hexagonal shaped SaZnO NPs with size range 30–40 nm. The synthesized SaZnO NPs reduced the growth and production of deoxynivalenol and zearalenone of F. graminearum in broth culture. Further analysis revealed that treatment of mycelia with SaZnO NPs resulted in the accumulation of ROS in the dose-dependent manner. Also, SaZnO NPs treatment enhanced lipid peroxidation, depleted ergosterol content, and caused detrimental damage to the membrane integrity of fungi. Moreover, SEM observations revealed that the presence of diverged micro-morphology (wrinkled, rough and shrank surface) in the macroconidia treated with SaZnO NPs. Taken together, SaZnO NPs may find a potential application in agriculture and food industries due to their potent antifungal activity.

Published in Frontiers in Microbiology

ISSN: 1664-302X (Online)
Publisher: Frontiers Media S.A.
Country of publisher: Switzerland
LCC subjects: Science: Microbiology
Website: http://www.frontiersin.org/journals/microbiology

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