Effect of titanium dioxide nanoparticles on zebrafish embryos and developing retina

Ya-Jie Wang; Zi-Zi He; Yang-Wu Fang; Yang Xu; Ya-Nan Chen; Guan-Qun Wang; Yong-Qiang Yang; Zhuo Yang; Yu-Hao Li

doi:10.3980/j.issn.2222-3959.2014.06.01

International Journal of Ophthalmology (Dec 2014)

Effect of titanium dioxide nanoparticles on zebrafish embryos and developing retina

Ya-Jie Wang,
Zi-Zi He,
Yang-Wu Fang,
Yang Xu,
Ya-Nan Chen,
Guan-Qun Wang,
Yong-Qiang Yang,
Zhuo Yang,
Yu-Hao Li

Affiliations

Ya-Jie Wang: Key Laboratory of Tumor Microenviroment and Neurovascular Regulation, Nankai University School of Medicine, Tianjin 300071, China
Zi-Zi He: Key Laboratory of Tumor Microenviroment and Neurovascular Regulation, Nankai University School of Medicine, Tianjin 300071, China
Yang-Wu Fang: Key Laboratory of Tumor Microenviroment and Neurovascular Regulation, Nankai University School of Medicine, Tianjin 300071, China
Yang Xu: State Key Laboratory of Medicinal Chemical Biology, College of Chemistry, Nankai University, Tianjin 300071, China
Ya-Nan Chen: Key Laboratory of Tumor Microenviroment and Neurovascular Regulation, Nankai University School of Medicine, Tianjin 300071, China
Guan-Qun Wang: Key Laboratory of Tumor Microenviroment and Neurovascular Regulation, Nankai University School of Medicine, Tianjin 300071, China
Yong-Qiang Yang: Key Laboratory of Tumor Microenviroment and Neurovascular Regulation, Nankai University School of Medicine, Tianjin 300071, China
Zhuo Yang: Key Laboratory of Tumor Microenviroment and Neurovascular Regulation, Nankai University School of Medicine, Tianjin 300071, China
Yu-Hao Li: Key Laboratory of Tumor Microenviroment and Neurovascular Regulation, Nankai University School of Medicine, Tianjin 300071, China

DOI: https://doi.org/10.3980/j.issn.2222-3959.2014.06.01
Journal volume & issue: Vol. 7, no. 6
pp. 917 – 923

Abstract

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AIM:To investigate the impact of titanium dioxide nanoparticles (TiO2 NPs) on embryonic development and retinal neurogenesis. METHODS:The agglomeration and sedimentation of TiO2 NPs solutions at different dilutions were observed, and the ultraviolet-visible spectra of their supernatants were measured. Zebrafish embryos were experimentally exposed to TiO2 NPs until 72h postfertilization (hpf). The retinal neurogenesis and distribution of the microglia were analyzed by immunohistochemistry and whole mount in situ hybridization. RESULTS: The1 mg/L was determined to be an appropriate exposure dose. Embryos exposed to TiO2 NPs had a normal phenotype. The neurogenesis was initiated on time, and ganglion cells, cones and rods were well differentiated at 72 hpf. The expression of fms mRNA and the 4C4 antibody, which were specific to microglia in the central nervous system (CNS), closely resembled their endogenous profile. CONCLUSION:These data demonstrate that short-term exposure to TiO2 NPs at a low dose does not lead to delayed embryonic development or retinal neurotoxicity.

Published in International Journal of Ophthalmology

ISSN: 2222-3959 (Print); 2227-4898 (Online)
Publisher: Press of International Journal of Ophthalmology (IJO PRESS)
Country of publisher: China
LCC subjects: Medicine: Ophthalmology
Website: http://www.ijo.cn/gjyken/ch/index.aspx

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