INFLUENCE OF POLYMYXIN B ON THE FORMATION OF BIOFILMS BY BACTERIUM Methylophilus quaylei ON POLYPROPYLENE AND TEFLON

Abstract

Here we examined the influence of polymyxin B, a lipopeptide antibiotic, on planktonic growth and biofilm of streptomycin-sensitive and streptomycin-resistant isogenic strains of bacteria Methylophilus quaylei on polypropylene and Teflon. Biofilm formation was quantified using crystal violet staining, determined by colony-forming unit and examined by light microscopy. It was found that of bacterial surface hydrophobicity affects the biofilm formation: biofilm formation of Methylophilus quaylei MT strain was better on such hydrophobic materials as Teflon and polypropylene. The minimum inhibitory concentration of polymyxin B for the used strains in planktonic and biofilm form was 1 μg/ml. The sublethal Polymyxin B in the concentration of 0.01 μg/ml stimulates biofilm formation and exhibits antibiofilm properties at the concentration of 0.10 μg/ml. Synergistic effect of polymyxin B and streptomycin on streptomycin-resistant strain M. quaylei SM was found.

Keywords

• bacterial biofilms
• polymyxin
• resistance to antibiotics
• methylotrophic bacteria