A Morphological and Biochemical Study of Ethanolic Fruit Extract of Berberis Vulgaris Against Gentamicin Induced Renal Damage in Albino Rats

Abstract

Objective: To analyze the reno protective potential of berberis vulgaris on renal function by measuring renal biomarkers (serum urea and serum creatinine). Study Design: Experimental lab-based study. Place and Duration of Study: Study was done in Baqai Medical & Dental University 4 July 2017 to 3 Aug 2017. Materials and Methods: Forty adult healthy male laboratory animals were randomly divided into four groups. Group A was treated as control group. Group B were administered with Berberis vulgaris fruit extract 100 mg/kg/body wt. single oral dose for 21 days. Group C were administered with gentamicin 100 mg/kg/day intraperitoneally single dose for 21 days. Group D, animals received gentamicin intraperitoneally 100mg/kg/day along with berberis vulgaris fruit extract orally for 21 days. Both kidneys were dissected, all sections obtained were stained with two dyes hematoxylin and eosin and periodic acid schiff stain for histomorphology. Standard error of mean was used to express results. Numerical data obtained from different groups were analyzed using SPSS version 21. P-value of 0.05 or less than 0.05 was considered statistically significant. Results: This study revealed that body weight, kidney weight has been reduced in gentamicin group whereas biomarkers and relative weight of kidney have shown significant increase in group D as compared to control group. Conclusion: Berberis vulgaris fruit extract ameliorates functional abnormalities along with biochemical parameters associated with gentamicin induced nephrotoxicity

Keywords

• berberis vulgaris
• gentamicin
• nephrotoxicity
• serum creatinine