Effets oestrogéniques du macéré aqueux des feuilles de Holarrhena floribunda (G. Don) Dur & Schinz chez la rate ovariectomisée

Abstract

Oestrogenic effects of macerated aqueous extracts of the leaves of Holarrhena fl oribunda (G. Don) Dur & Schinz on ovariectomized rat. Estrogenic effects of aqueous extracts of leaves of Holarrhena fl oribunda have been evaluated by uterotrophic assay. Ovariectomized mice were used and treated subcutaneously during seven days with 50, 100, 200 mg.kg-1 doses of Holarrhena fl oribunda and with 100, 200 mg.kg-1 doses of Holarrhena fl oribunda plus 25 mg.kg-1 of oestradiol 17-β. Estrogenic activity was analysed by uterine dry and wet weight, surrenal gland wet weight, vaginal opening, protein and cholesterol level in uteri horn. Administration of extracts to the doses of 100 and 200 mg.kg-1 exhibits increase of uterine dry and wet weight, surrenal gland wet weight, vaginal opening, protein levels and decrease of level of cholesterol in uterine horn. The results suggest that, aqueous extracts of Holarrhena fl oribunda possesses estrogenic type effect. But when 100 and 200 mg.kg-1 of Holarrhena fl oribunda were given alone with 25 mg.kg-1 of estradiol 17-β, the estrogenic effect was slight. These results supposed that H. fl oribunda is a weak phytoestrogen and the aqueous extracts of the leaves behad as partial agonistic

Keywords

• Holarrhena fl oribunda
• estrogenics effects
• uteri
• ovariectomized rat