Molecules (Oct 2013)

Direct Light-up of cAMP Derivatives in Living Cells by Click Reactions

  • Yan Xu,
  • Hongshan Liu,
  • Tetsuya Hayashi,
  • Makoto Komiyama,
  • Kenichiro Ito

DOI
https://doi.org/10.3390/molecules181012909
Journal volume & issue
Vol. 18, no. 10
pp. 12909 – 12915

Abstract

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8-Azidoadenosine 3′,5′-cyclic monophosphate (8-azido cAMP) was directly detected in living cells, by applying Cu-free azide-alkyne cycloaddition to probe cAMP derivatives by fluorescence light-up. Fluorescence emission was generated by two non-fluorescent molecules, 8-azido cAMP as a model target and difluorinated cyclooctyne (DIFO) reagent as a probe. The azide-alkyne cycloaddition reaction between 8-azido cAMP and DIFO induces fluorescence in 8-azido cAMP. The fluorescence emission serves as a way to probe 8-azido cAMP in cells.

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