miR-195 targeting MYB activates the PI3K-Akt pathway to regulate the proliferation and malignant metastasis of squamous cell carcinoma of the skin

Abstract

Objective: To investigate the effect and mechanism of miR-195 on the proliferation and malignant metastasis of squamous cell carcinoma of the skin. Methods: The expressions of miR-195 and MYB gene in the epidermoid carcinoma cell line (A431 cells) and the human epidermal keratinocyte line (HaCaT cells) were detected by RT-qPCR. The effects of up-regulation and down-regulation of miR-195 on the proliferation ability of A431 cells were detected by CCK-8 experiment. The effects of up-regulation and down-regulation of miR-195 on the expression of apoptosis related proteins Bax and Bcl-2 in A431 cells were detected by Western blot. The effects of up-regulation and down-regulation of miR-195 on EMT of A431 cells were detected by Western blot. The relationship between miR-195 and MYB was analyzed by TargetScan, and the effects of down-regulation of MYB on A431 cell proliferation and EMT were analyzed by dual luciferase reporter gene assay. Western blot was used to detect the effects of MYB on PI3K-Akt pathway. Results: Compared with the expressions of miR-195 and MYB in HaCaT cells, the expressions of miR-195 in A431 cells were significantly down-regulated (P<0.01), the expressions of MYB were significantly up-regulated (P<0.01). Up-regulation of miR-195 inhibited the proliferation and EMT of A431 cells and promoted apoptosis, while down-regulation of miR-195 produced an opposite effect. miR-195 and MYB had a targeted and negative regulatory relationship. Down-regulation of MYB inhibited A431 cell proliferation and EMT. Overexpression of MYB activated the PI3K-Akt pathway and promoted proliferation and EMT of A431 cells. Conclusion: miR-195 regulates the proliferation and metastasis of A431 cells by targeting the MYB to activate the PI3K-Akt pathway.

Keywords

• mir-195
• myb
• pi3k-akt
• squamous cell carcinoma
• proliferation