Isolation and characterization of Bifidobacteria from broiler crops and the possibility to use as probiotics

Abstract

Abstract The aim of this study is to isolate Bifidobacteria from broiler crops to obtain bacteria having the ability of adhesion to digestive canal and good activity; The isolated bacteria were passaged three serial passages in chicks for 6 days interval, after that, Three isolates were selected and testing for probiotic and biochemical properties on them (api 20 A test one of biochemical tests which was conducted on one isolate only). These isolates were showed same properties, they were unable to grow in aerobic conditions, they couldn't produce catalase enzyme and CO2, they could produce acid, and analyze starch. They had high ability for adhesion on crop epithelial cells, and they were unable to grow in acid medium (pH<4.5) but they were able to growth in medium (pH=6). On other hand, the isolates were sensitive for most antibiotics. By using the api 20 A test, one of these isolates showed similar characters to Bifidobacterium adolescentis and possibly could be used as probiotic for broiler.

Keywords

• bifidobacteria
• broiler crops
• probiotics
• characterization