Evaluation of crossmatch techniques for deceased donor transplant program – Need of the hour

Abstract

Background and Objective: It is important to strengthen deceased donor organ transplant program to help the patients who do not have living kidney donor available. However, there are lots of challenges in deceased donor program. There are challenges to convince families of brain dead patients for organ donation, but transportation of organ as well as allocation is also difficult. One of the main challenges for transplant immunology laboratory is correct crossmatch technique which is sensitive as well as quick and easy to perform. The aim of this study is to evaluate the results of Luminex lysate donor-specific antibody (DSA) crossmatch (LumCross-match) testing for deceased donor renal transplant cases and correlate with graft dysfunction. Methods: We use Luminex lysate DSA crossmatch for human leukocyte antigen testing for deceased donor renal transplants. In 2 years, we had 177 deceased donations and 1184 crossmatches were done. Results: Total 342 patients received kidneys in 2 years. No hyperacute rejection was reported though 28 cases had signs of antibody-mediated rejection posttransplant. Conclusion: We found Luminex lysate crossmatch useful in deceased donor transplant program because of easy procedure, high throughput, more sensitive, and less laborious as well as for posttransplant monitoring.

Keywords

• deceased donor
• luminex lysate crossmatch
• renal transplant