Frontiers in Pharmacology (Jul 2022)

Therapeutic Effect of Ultrasound Combined With Porous Lipid Clioquinol/PLGA Microbubbles on Ferroptosis in HL-1 Cardiac Cell Induced by Isoproterenol Attack

  • Nana Li,
  • Lei Dong,
  • Yuanyuan Shen,
  • Yongling Wang,
  • Liansheng Chang,
  • Hongwei Wu,
  • Yuqiao Chang,
  • Menghao Li,
  • Dan Li,
  • Zhaoyi Li,
  • Mei He,
  • Cheng Li,
  • Yao Wei,
  • Haiqin Xie,
  • Feng Wang

DOI
https://doi.org/10.3389/fphar.2022.918292
Journal volume & issue
Vol. 13

Abstract

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In recent years, studies have shown a close relationship between cardiomyocyte death and ferroptosis. Clioquinol (CQ) can inhibit ferroptosis. Porous lipid-poly (lactic-co-glycolic acid) (PLGA) microbubbles (MBs) were prepared by double emulsification (W1/O/W2) using 1,2-dioctadecanoyl-sn-glycero-3-phophocholine and PLGA as raw materials. Porous lipid-PLGA MBs were used as carriers to prepare CQ/PLGA MBs containing CQ. CQ/PLGA had the advantages of high drug loading, good biocompatibility, and sustained release. Our results showed that CQ/PLGA improved the effect of CQ and reduced its cytotoxicity. Under low-frequency ultrasound with certain parameters, CQ/PLGA showed steady-state cavitation, which increased the membrane permeability of mouse cardiomyocyte HL-1 to a certain extent and further prevented the process of ferroptosis in mouse cardiomyocyte HL-1.

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