Nature Communications (Oct 2018)
Improved methods for marking active neuron populations
- Benjamien Moeyaert,
- Graham Holt,
- Rajtarun Madangopal,
- Alberto Perez-Alvarez,
- Brenna C. Fearey,
- Nicholas F. Trojanowski,
- Julia Ledderose,
- Timothy A. Zolnik,
- Aniruddha Das,
- Davina Patel,
- Timothy A. Brown,
- Robert N. S. Sachdev,
- Britta J. Eickholt,
- Matthew E. Larkum,
- Gina G. Turrigiano,
- Hod Dana,
- Christine E. Gee,
- Thomas G. Oertner,
- Bruce T. Hope,
- Eric R. Schreiter
Affiliations
- Benjamien Moeyaert
- Howard Hughes Medical Institute
- Graham Holt
- Howard Hughes Medical Institute
- Rajtarun Madangopal
- Neuronal Ensembles in Addiction Section, Behavioral Neuroscience Research Branch, Intramural Research Program, National Institute on Drug Abuse, National Institutes of Health
- Alberto Perez-Alvarez
- Institute for Synaptic Physiology, University Medical Center Hamburg-Eppendorf
- Brenna C. Fearey
- Institute for Synaptic Physiology, University Medical Center Hamburg-Eppendorf
- Nicholas F. Trojanowski
- Department of Biology, Brandeis University
- Julia Ledderose
- Institute of Biochemistry, Charité - Universitätsmedizin Berlin
- Timothy A. Zolnik
- NeuroCure Cluster of Excellence, Department of Biology, Humboldt University
- Aniruddha Das
- Department of Neurosciences, Lerner Research Institute, Cleveland Clinic Foundation
- Davina Patel
- Department of Neurosciences, Lerner Research Institute, Cleveland Clinic Foundation
- Timothy A. Brown
- Howard Hughes Medical Institute
- Robert N. S. Sachdev
- NeuroCure Cluster of Excellence, Department of Biology, Humboldt University
- Britta J. Eickholt
- Institute of Biochemistry, Charité - Universitätsmedizin Berlin
- Matthew E. Larkum
- NeuroCure Cluster of Excellence, Department of Biology, Humboldt University
- Gina G. Turrigiano
- Department of Biology, Brandeis University
- Hod Dana
- Howard Hughes Medical Institute
- Christine E. Gee
- Institute for Synaptic Physiology, University Medical Center Hamburg-Eppendorf
- Thomas G. Oertner
- Institute for Synaptic Physiology, University Medical Center Hamburg-Eppendorf
- Bruce T. Hope
- Neuronal Ensembles in Addiction Section, Behavioral Neuroscience Research Branch, Intramural Research Program, National Institute on Drug Abuse, National Institutes of Health
- Eric R. Schreiter
- Howard Hughes Medical Institute
- DOI
- https://doi.org/10.1038/s41467-018-06935-2
- Journal volume & issue
-
Vol. 9,
no. 1
pp. 1 – 12
Abstract
Methods to directly label active neurons are still lacking. Here the authors develop CaMPARI2, a photoconvertible fluorescent protein sensor for neuronal activity with improved brightness and calcium binding kinetics, as well as an antibody to amplify the activated sensor signal in fixed samples.
