iScience (Jul 2021)

Comparative analysis of clonal evolution among patients with right- and left-sided colon and rectal cancer

  • Santasree Banerjee,
  • Xianxiang Zhang,
  • Shan Kuang,
  • Jigang Wang,
  • Lei Li,
  • Guangyi Fan,
  • Yonglun Luo,
  • Shuai Sun,
  • Peng Han,
  • Qingyao Wu,
  • Shujian Yang,
  • Xiaobin Ji,
  • Yong Li,
  • Li Deng,
  • Xiaofen Tian,
  • Zhiwei Wang,
  • Yue Zhang,
  • Kui Wu,
  • Shida Zhu,
  • Lars Bolund,
  • Huanming Yang,
  • Xun Xu,
  • Junnian Liu,
  • Yun Lu,
  • Xin Liu

Journal volume & issue
Vol. 24, no. 7
p. 102718

Abstract

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Summary: Tumor multiregion sequencing reveals intratumor heterogeneity (ITH) and clonal evolution playing a key role in tumor progression and metastases. Large-scale high-depth multiregional sequencing of colorectal cancer, comparative analysis among patients with right-sided colon cancer (RCC), left-sided colon cancer (LCC), and rectal cancer (RC), as well as the study of lymph node metastasis (LN) with extranodal tumor deposits (ENTDs) from evolutionary perspective remain weakly explored. Here, we recruited 68 patients with RCC (18), LCC (20), and RC (30). We performed high-depth whole-exome sequencing of 206 tumor regions including 176 primary tumors, 19 LN, and 11 ENTD samples. Our results showed ITH with a Darwinian pattern of evolution and the evolution pattern of LCC and RC was more complex and divergent than RCC. Genetic and evolutionary evidences found that both LN and ENTD originated from different clones. Moreover, ENTD was a distinct entity from LN and evolved later.

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