Substitution of the Methionine Axial Ligand of the T1 Copper for the Fungal-like Phenylalanine Ligand (M298F) Causes Local Structural Perturbations that Lead to Thermal Instability and Reduced Catalytic Efficiency of the Small Laccase from Streptomyces coelicolor A3(2)
Kairit Zovo,
Hegne Pupart,
Arie Van Wieren,
Richard E. Gillilan,
Qingqiu Huang,
Sudipta Majumdar,
Tiit Lukk
Affiliations
Kairit Zovo
Department of Chemistry and Biotechnology, Tallinn University of Technology, Tallinn, Estonia
Hegne Pupart
Department of Chemistry and Biotechnology, Tallinn University of Technology, Tallinn, Estonia
Arie Van Wieren
Department of Chemistry, Biochemistry, Physics and Engineering, Indiana University of Pennsylvania, Indiana, Pennsylvania, United States
Richard E. Gillilan
MacCHESS (Macromolecular Diffraction Facility at CHESS), Cornell University, Ithaca, New York, United States
Qingqiu Huang
MacCHESS (Macromolecular Diffraction Facility at CHESS), Cornell University, Ithaca, New York, United States
Sudipta Majumdar
Department of Chemistry, Biochemistry, Physics and Engineering, Indiana University of Pennsylvania, Indiana, Pennsylvania, United States
Tiit Lukk
Department of Chemistry and Biotechnology, Tallinn University of Technology, Tallinn, Estonia
