Preliminary investigation and antimicrobial screening of successive extracts of phytoconstituents from Cassia fistula of Haridwar region, India

Abstract

Cassia fistula belongs to family leguminosae. It is a medium sized tree and its different parts are used in Ayurvedic medicine as well as in home remedies for common ailments. The plant is easily available in Haridwar region. The phytoconstituents of a same plant vary from region to region. In the present study bark of Cassia fistula is used. The material was collected (in Haridwar region, India), dried in shade; powdered and extracted successively with different solvents in an increasing order of polarity. Phytochemical investigation was performed using different identification tests. The different extracts were also screened for antimicrobial activity. For which both Gram positive and Gram negative bacterial strain were selected. Antimicrobial test was performed by agar well diffusion method. All the tests were performed in a triplicate. The different phytoconstituents present in the bark extract are responsible for such an appreciable activity against selected pathogens.

Keywords

• Cassia fistula
• successive extraction
• phytochemical investigation
• antimicrobial screening